VSL#3 probiotics exerts the anti-inflammatory activity via PI3k/Akt and NF-κB pathway in rat model of DSS-induced colitis
VSL#3 probiotics exerts the anti-inflammatory activity via PI3k/Akt and NF-κB pathway in rat model of DSS-induced colitis
复制标题
DOI：
10.1007/s11010-012-1488-3
复制
发表时间：
2013-02-01
影响因子：
4.3
通讯作者：
Jiang, Min
中科院分区：
文献类型：
--
作者：
Dai, Cong;Zheng, Chang-Qing;Jiang, Min
VSL#3益生菌可有效诱导和维持临床溃疡性结肠炎的缓解。然而，其机制并不完全清楚。本研究旨在检查VSLI #3益生菌对葡聚糖硫酸钠（DSS）诱导的大鼠结肠炎的影响。大鼠连续7天给予DSS 3.5%诱导急性结肠炎。诱导结肠炎后，两组大鼠通过胃管接受15 mg VSLI #3或安慰剂给药，每日一次;诱导结肠炎后，另外两组大鼠通过腹腔内注射接受渥曼青霉素（1 mg/kg）或渥曼青霉素+VSLI #3给药。通过髓过氧化物酶（MPO）活性评估抗炎活性。评估结肠组织中炎症相关介质（iNOS、考克斯-2、NF-κ B、Akt和p-Akt）和细胞因子（TNF-α、IL-6和IL-10）的表达。还测量了TNF-α、IL-6和IL-10血清水平。我们的结果表明，VSL#3和渥曼青霉素通过降低疾病活动指数和MPO活性具有抗炎特性。此外，VSLI #3和渥曼青霉素给药7天导致结肠组织中iNOS、考克斯-2、NF-κ B、TNF-α、IL-6和p-Akt减少，IL-10表达增加。同时，VSLI #3和渥曼青霉素给药导致TNF-α和IL-6降低，IL-10血清水平升高。VSLI #3益生菌治疗通过抑制PI 3 K/Akt和NF-κ B通路在DSS诱导的结肠炎大鼠模型中发挥抗炎活性。
VSL#3 probiotics can be effective on induction and maintenance of the remission of clinical ulcerative colitis. However, the mechanisms are not fully understood. The aim of this study was to examine the effects of VSL#3 probiotics on dextran sulfate sodium (DSS)-induced colitis in rats. Acute colitis was induced by administration of DSS 3.5 % for 7 days in rats. Rats in two groups were treated with either 15 mg VSL#3 or placebo via gastric tube once daily after induction of colitis; rats in other two groups were treated with either the wortmannin (1 mg/kg) via intraperitoneal injection or the wortmannin + VSL#3 after induction of colitis. Anti-inflammatory activity was assessed by myeloperoxidase (MPO) activity. Expression of inflammatory related mediators (iNOS, COX-2, NF-kappa B, Akt, and p-Akt) and cytokines (TNF-alpha, IL-6, and IL-10) in colonic tissue were assessed. TNF-alpha, IL-6, and IL-10 serum levels were also measured. Our results demonstrated that VSL#3 and wortmannin have anti-inflammatory properties by the reduced disease activity index and MPO activity. In addition, administration of VSL#3 and wortmannin for 7 days resulted in a decrease of iNOS, COX-2, NF-kappa B, TNF-alpha, IL-6, and p-Akt and an increase of IL-10 expression in colonic tissue. At the same time, administration of VSL#3 and wortmannin resulted in a decrease of TNF-alpha and IL-6 and an increase of IL-10 serum levels. VSL#3 probiotics therapy exerts the anti-inflammatory activity in rat model of DSS-induced colitis by inhibiting PI3K/Akt and NF-kappa B pathway.