Incorporation of label from acetate and laurate into the mannan of Leishmania donovani via the glyoxylate cycle.
Incorporation of label from acetate and laurate into the mannan of Leishmania donovani via the glyoxylate cycle.
复制标题
通过乙醛酸循环将乙酸盐和月桂酸盐的标记掺入杜氏利什曼原虫的甘露聚糖中。
DOI：
10.1111/j.1550-7408.1993.tb04467.x
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Blum,JJ
中科院分区：
文献类型：
--
作者：
Keegan,FP;Blum,JJ
停滞后期的杜氏利什曼原虫将[2-14C]乙酸酯和[1-14C]月桂酸酯的标记结合到甘露聚糖残基中，从而证实了这些寄生原虫中存在功能上的乙醛酸旁路。在缓冲液中孵育1小时期间，分离的洗涤的呼叫还将来自[2-14C]乙酸酯和[1-14C]月桂酸酯的标签结合到甘露聚糖中。葡萄糖对标记物掺入甘露聚糖没有影响，但谷氨酸使[2-14C]乙酸酯和[1-14C]月桂酸酯的标记物掺入量分别增加了4倍和2.4倍。星形孢子素是一种抑制谷氨酸和丙氨酸氧化的蛋白激酶抑制剂，但不能抑制从[2-14C]乙酸酯到甘露聚糖的标记掺入。高渗透压可抑制约33%的标记物掺入甘露聚糖。这些结果表明，乙醛酸循环和/或随后从果糖-6-磷酸到甘露聚糖的生物合成途径受到调控。
Leishmania donovanipromastigotes in late‐stationary phase incorporated label from [2‐14C]acetate and [1‐14C]laurate into the mannose residues of mannan, thus confirming the presence of a functional glyoxylate bypass in these parasitic protozoa. Isolated, washed calls also incorporated label from [2‐14C]acetate and [1‐14C]laurate into mannan during a 1‐hr incubation in buffer. Glucose had no effect on label incorporation into mannan, but glutamate caused over a four‐fold increase in incorporation from [2‐14C]acetate and a 2.4‐fold increase from [1‐14C]laurate. Staurosporine, a protein kinase inhibitor that inhibits glutamate and alanine oxidation, did not inhibit label incorporation from [2‐14C]acetate into mannan. Hyperosmolality caused about a 33% inhibition of label incorporation into mannan. These results show the glyoxylate cycle and/or the subsequent biosynthetic pathway from fructose‐6‐phosphate to mannan are subject to regulation.