Neuropathic changes in equine laminitis pain
Neuropathic changes in equine laminitis pain
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DOI：
10.1016/j.pain.2007.08.035
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发表时间：
2007-12-05
期刊：
影响因子：
7.4
通讯作者：
Fleetwood-Walker, Susan M.
中科院分区：
文献类型：
--
作者：
Jones, Emma;Vinuela-Fernandez, Ignacio;Fleetwood-Walker, Susan M.
蹄板炎是一种常见的使马衰弱的疾病，涉及蹄板层真皮-表皮交界处的痛苦破坏。这种情况通常对传统的抗炎镇痛是难治的，并导致持续的疼痛，在严重的情况下需要安乐死。利用行为性疼痛指标的量化，结合支配蹄部的周围神经的组织学研究，研究了足板炎疼痛的机制。与正常马相比，Laminitic马表现出持续改变或异常的行为，例如前肢抬起的增加和在箱子后面花费的时间比例增加。纹层状马指神经的电子显微图分析显示，与正常马相比，周围神经形态异常，无髓鞘纤维（43.2%）和有髓鞘纤维（34.6%）数量减少。支配蹄的感觉神经细胞体，颈C8背根神经节（DRG）中神经元损伤标志物，激活转录因子-3 （ATF3）在nf -200免疫阳性的大神经元和外周血素或ib4阳性的小神经元中表达上调。有髓鞘传入神经元中神经肽Y （NPY）的表达也显著增加。这些变化与其他神经性疼痛状态的变化相似，在板层状马的C4 DRG中没有观察到，这与前肢的神经支配无关。这项研究为与马板炎相关的慢性疼痛状态的神经性成分提供了新的证据，表明抗神经性镇痛治疗可能在这种情况的治疗中发挥作用。(c) 2007年国际疼痛研究协会。Elsevier B.V.版权所有。
Laminitis is a common debilitating disease in horses that involves painful disruption of the lamellar dermo-epidermal junction within the hoof. This condition is often refractory to conventional anti-inflammatory analgesia and results in unremitting pain, which in severe cases requires euthanasia. The mechanisms underlying pain in laminitis were investigated using quantification of behavioural pain indicators in conjunction with histological studies of peripheral nerves innervating the hoof. Laminitic horses displayed consistently altered or abnormal behaviours such as increased forelimb lifting and an increased proportion of time spent at the back of the box compared to normal horses. Electron micrographic analysis of the digital nerve of laminitic horses showed peripheral nerve morphology to be abnormal, as well as having reduced numbers of unmyelinated (43.2%) and myelinated fibers (34.6%) compared to normal horses. Sensory nerve cell bodies innervating the hoof, in cervical, C8 dorsal root ganglia (DRG), showed an upregulated expression of the neuronal injury marker, activating transcription factor-3 (ATF3) ill both large NF-200-immunopositive neurons and small neurons that were either peripherin- or IB4-positive. A significantly increased expression of neuropeptide Y (NPY) was also observed in myelinated afferent neurons. These changes are similar to those reported in other neuropathic pain states and were not observed in the C4 DRG of laminitic horses, which is not associated with innervation of the forelimb. This study provides novel evidence for a neuropathic component to the chronic pain state associated with equine laminitis, indicating that anti-neuropathic analgesic treatment may well have a role in the management of this condition. (c) 2007 International Association for the Study of Pain. Published by Elsevier B.V. All rights reserved.