Cancer-upregulated gene 2 (CUG2) overexpression induces apoptosis in SKOV-3 cells
Cancer-upregulated gene 2 (CUG2) overexpression induces apoptosis in SKOV-3 cells
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DOI：
10.1002/cbf.1678
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发表时间：
2010-08-01
影响因子：
3.6
通讯作者：
Lee, Soojin
中科院分区：
文献类型：
--
作者：
Lee, Sunhee;Koh, Wansoo;Lee, Soojin
癌症上调基因2（CUG 2）最初被鉴定为在多种人类癌症中普遍上调的潜在癌基因。最近，CUG 2也被确定为一个新的成员的着丝粒蛋白复合物，重要的功能动粒复合物的形成。目前，我们报告了当CUG 2基因在SKOV-3人卵巢癌细胞系中过表达时，该基因的促凋亡作用。通过细胞活力测定、流式细胞术分析、染色体片段化测定和死亡底物聚（ADP-核糖）聚合酶的裂解，独立地证明了由CUG 2过表达介导的细胞凋亡。此外，半胱天冬酶-3和-8的活化和线粒体细胞色素c的胞质易位在CUG 2表达后是明显的。在斑马鱼胚胎中过表达CUG 2时，在斑马鱼早期发育过程中也观察到细胞凋亡。我们提出CUG 2的高表达诱导凋亡性细胞死亡。版权所有（C）2010约翰威利父子有限公司
Cancer-upregulated gene 2 (CUG2) was originally identified as a potential oncogene commonly up-regulated in various human cancers. Recently, CUG2 was also identified as a new member of a centromere protein complex, important in the formation of a functional kinetochore complex. Presently, we report the pro-apoptotic effect of CUG2 when this gene was overexpressed in the SKOV-3 human ovarian cancer cell line. Apoptotic cell death mediated by CUG2 overexpression was independently demonstrated using cell viability determination, flow cytometry analysis, chromosome fragmentation assay, and the cleavage of the death substrate poly(ADP-ribose) polymerase. Moreover, activation of caspase-3 and -8 and the cytoplasmic translocation of mitochondrial cytochrome c were evident upon CUG2 expression. Apoptotic cell death was also observed during early development of zebrafish when CUG2 was overexpressed in zebrafish embryo. We propose that high expression of CUG2 induces apoptotic cell death. Copyright (C) 2010 John Wiley & Sons, Ltd.