A single bout of cycling exercise induces nucleosome repositioning in the skeletal muscle of lean and overweight/obese individuals.
A single bout of cycling exercise induces nucleosome repositioning in the skeletal muscle of lean and overweight/obese individuals.
复制标题
DOI：
10.1111/dom.14541
复制
发表时间：
2022-01
期刊：
影响因子：
--
通讯作者：
Henagan TM
中科院分区：
文献类型：
--
作者：
Devarshi PP;Pereyra AS;Ellis JM;Henagan TM
急性有氧运动改善，而超重损害，骨骼肌线粒体功能与胰岛素敏感性降低相关。在此，在年龄为19-30岁的男性中比较了单次运动对骨骼肌的分子和代谢影响，这些男性要么是瘦的（BMI<25，18.5-24.1 kg/m2，n=15），要么是超重/肥胖的（Ov/Ob; BMI≥25，25.5-36.9 kg/m2，n=15）。高碳水化合物早餐（7千卡/千克; 60%碳水化合物，25%脂肪，15%蛋白质）后4小时，参与者进行自行车运动（50%VO2max，消耗约650千卡）。在饭前30分钟和运动后立即采集肌肉活检和外周血样本。肌肉长链酰基肉毒碱增加Ov/Ob相比，瘦，有或没有运动。一个单一的运动回合增加基因的mRNA丰度与线粒体和胰岛素信号在瘦和Ov/Ob。通过微球菌核酸酶消化和深度测序（MNase-seq）进行的核小体作图显示，在瘦和Ov/Ob中，运动将− 1 N核小体从PGC 1a启动子和其他线粒体基因的转录起始位点重新定位，但不影响与胰岛素信号相关的基因。总之，这些数据表明，一个单一的运动回合诱导骨骼肌的表观遗传学改变的BMI独立的方式。
Acute aerobic exercise improves, whereas being overweight impairs, skeletal muscle mitochondrial function in correlation with reduced insulin sensitivity. Here, the molecular and metabolic effects of a single exercise bout in the skeletal muscle was compared in men, aged 19-30 years, that were either lean (BMI<25, 18.5-24.1 kg/m2, n=15) or overweight/obese (Ov/Ob; BMI≥25, 25.5-36.9 kg/m2, n=15). Four hours after a high-carbohydrate breakfast (7 kcal/kg; 60% carbohydrate, 25% fat, 15% protein), participants performed cycling exercise (50% VO2max, expending ~650 kcal). Muscle biopsies and peripheral blood samples were collected 30 minutes before the meal and immediately after exercise. Muscle long-chain acylcarnitines were increased in Ov/Ob compared to lean, with or without exercise. A single exercise bout increased mRNA abundance of genes related to mitochondria and insulin signaling in both lean and Ov/Ob. Nucleosome mapping by micrococcal nuclease digestion with deep sequencing (MNase-seq) revealed that exercise repositioned the −1N nucleosome away from the transcription start site of the PGC1a promoter and of other mitochondrial genes, but did not affect genes related to insulin signaling, in both lean and Ov/Ob. In conclusion, these data suggest that a single exercise bout induced epigenetic alterations in skeletal muscle in a BMI-independent manner.
登录
查看更多内容
影响因子：
7.3
作者：
Henagan, Tara M.;Stefanska, Barbara;Devarshi, Prasad P.
通讯作者：
Devarshi, Prasad P.
影响因子：
29
作者：
Barres, Romain;Yan, Jie;Zierath, Juleen R.
通讯作者：
Zierath, Juleen R.
影响因子：
29
作者：
Barres, Romain;Osler, Megan E.;Zierath, Juleen R.
通讯作者：
Zierath, Juleen R.
影响因子：
7.2
作者：
Bordenave, S.;Brandou, F.;Brun, J. -F.
通讯作者：
Brun, J. -F.
影响因子：
7.7
作者：
DEVLIN, JT;HIRSHMAN, M;HORTON, ES
通讯作者：
HORTON, ES