Computational Modeling of Cell Growth Heterogeneity in a Perfused 3D Scaffold
Computational Modeling of Cell Growth Heterogeneity in a Perfused 3D Scaffold
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DOI：
10.1021/ie900418g
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发表时间：
2010-01-20
影响因子：
4.2
通讯作者：
Elvassore, Nicola
中科院分区：
文献类型：
--
作者：
Flaibani, Marina;Magrofuoco, Enrico;Elvassore, Nicola
我们的目标是建立一个计算模型，描述细胞在灌注生物反应器中生长过程中三维多孔支架内细胞异质性的时空演变。假设支架由具有限定直径分布的独立平行圆柱形通道的集合体形成。每个通道中的总流量分配取决于油的有效直径，该有效直径被通道壁的细胞生长油减小。解决了一种代谢物的质量平衡和细胞体积平衡。对于每个通道直径，文件模型模拟提供了速度、剪切应力、代谢物浓度和细胞体积生长的时空演变。特别地，所有这些结果都可以作为通道直径的函数进行分析，从而提供细胞特性异质性的所有评价。该模型描述了细胞生长在每个通道直径中可以是实质上不同的。例如，在小直径通道中，细胞生长受到代谢物质量传递的限制，而在较大直径通道中，剪切应力抑制细胞生长。该数学模型可能是一个重要的工具，先验估计的时间变化的细胞体积分数和其程度的异质性作为一个功能的操作参数和支架孔径分布。
Our goal was to develop a computational Model describing the spatiotemporal evolution of cell heterogeneity within a three-dimensional Porous Scaffold during cell growth in a perfusion bioreactor. The scaffold was assumed formed by an ensemble of independent parallel cylindrical channels with a defined diameter distribution. The total flow rate partitioning in each channel depends oil the effective diameter, which is reduced by the cell growth oil the channel wall. The mass balance for one metabolite and the cell volume balance were solved. For each channel diameter, file model simulation provide the spatiotemporal evolution of velocity, shear stress, metabolite concentration, and cell Volume growth. In particular, all of these outcomes can be analyzed as a function of channel diameter providing ail evaluation of cell property heterogeneity. The model describes that the cell growth can be Substantially different in each channel diameter. For instance, in file small diameter channel, cell growth is limited by metabolite mass transport, whereas in the larger diameter channel, shear stress inhibits cell growth. This mathematical model Could be an important tool for a priori estimation of the time variation of the cell volume fraction and its degree of heterogeneity as a function of operational parameters and scaffold pore size distribution.