Programmed cell death remodels lace plant leaf shape during development
Programmed cell death remodels lace plant leaf shape during development
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DOI：
10.1105/tpc.016188
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发表时间：
2004-01-01
期刊：
影响因子：
11.6
通讯作者：
Dengler, NG
中科院分区：
文献类型：
--
作者：
Gunawardena, AHLAN;Greenwood, JS;Dengler, NG
程序性细胞死亡（PCD）在高等植物的叶形发育重塑中起作用，这一过程类似于脊椎动物肢体中的趾形成。在这项研究中，我们提供了一个细胞学表征的时间过程中的事件作为PCD重塑年轻的花边植物扩展叶。液泡膜破裂是该系统中的第一个PCD事件，其表现为细胞质流动的改变、花青素颜色的丧失和超微结构的外观。细胞核成为末端脱氧核苷酸转移酶介导的dUTP缺口末端标记阳性后不久，但不成为形态学改变，直到后期的PCD。基因组DNA被片段化，但不成核小体间单位。其他细胞质变化，如细胞器的收缩和降解，发生较晚。这种形式的PCD类似于细胞学执行中的气管元件分化，但需要独特的发育调节，使得位于静脉之间等距的组织的离散面板经历PCD，而周围的细胞不经历PCD。
Programmed cell death (PCD) functions in the developmental remodeling of leaf shape in higher plants, a process analogous to digit formation in the vertebrate limb. In this study, we provide a cytological characterization of the time course of events as PCD remodels young expanding leaves of the lace plant. Tonoplast rupture is the first PCD event in this system, indicated by alterations in cytoplasmic streaming, loss of anthocyanin color, and ultrastructural appearance. Nuclei become terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling positive soon afterward but do not become morphologically altered until late stages of PCD. Genomic DNA is fragmented, but not into internucleosomal units. Other cytoplasmic changes, such as shrinkage and degradation of organelles, occur later. This form of PCD resembles tracheary element differentiation in cytological execution but requires unique developmental regulation so that discrete panels of tissue located equidistantly between veins undergo PCD while surrounding cells do not.