The tomato SlWRKY gene plays an important role in the regulation of defense responses in tobacco
The tomato SlWRKY gene plays an important role in the regulation of defense responses in tobacco
复制标题
番茄SlWRKY基因在烟草防御反应调节中发挥重要作用
DOI：
10.1016/j.bbrc.2012.09.120
复制
发表时间：
2012-10-26
影响因子：
3.1
通讯作者：
Jin, Hui
中科院分区：
文献类型：
--
作者：
Li, Jing-bin;Luan, Yu-shi;Jin, Hui
WRKY类转录因子参与植物生长发育和逆境应答的多个方面。SlWRKY基因是从番茄（Solanum lycopersicum L.）中克隆到的一个cDNA克隆，编码552个氨基酸，具有WRKY蛋白家族Ⅰ组蛋白的结构特征。cv中薯4号）进行同源克隆。半定量RT-PCR分析表明，盐和干旱处理均上调了番茄幼苗的SlWRKY基因表达。为了研究SlWRKY的生物学作用，我们产生了过表达SlWRKY的转基因烟草，并分析了它们对盐和干旱胁迫的响应。转基因烟草植株比野生型植株表现出更旺盛的生长，并表现出对盐和干旱胁迫的高耐受性。为了减轻氧化损伤，转基因烟草叶片中抗氧化酶活性增加，EC和MDA含量降低。此外，观察到SlWRKY蛋白调节下游基因并增加防御相关的PR 1和PR 2基因的表达。这些结果表明，SlWRKY在响应非生物胁迫中起着重要作用。(C)2012 Elsevier Inc. All rights reserved.
WRKY-type transcription factors are involved in multiple aspects of plant growth, development and stress responses. SlWRKY, a cDNA clone encoding a polypeptide of 552 amino acids and exhibiting the structural features of group I of WRKY protein family, was isolated from tomato (Solanum lycopersicum L. cv Zhongshu No. 4) using the homologous cloning method. Semi-quantitative RT-PCR analysis indicated that SlWRKY was up-regulated by salt and drought treatment in tomato seedlings. To investigate the biological roles of SlWRKY, we generated transgenic tobaccos overexpressing the SlWRKY and analyzed their responses to salt and drought stresses. Transgenic tobacco plants exhibited more vigorous growth than wild-type plants and display high tolerance to salt and drought stresses. In order to minimize oxidative damage, the activities of antioxidant enzymes were increased but EC and the MDA content were decreased in the transgenic tobacco leaves. Furthermore, it was observed that the SlWRKY proteins regulate the downstream genes and increased the expression of defense-related PR1 and PR2 genes. These results demonstrate that, SlWRKY plays an important role in responding to abiotic stress. (C) 2012 Elsevier Inc. All rights reserved.