Expression of gastric gland mucous cell-type mucin in normal and neoplastic human tissues
Expression of gastric gland mucous cell-type mucin in normal and neoplastic human tissues
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DOI：
10.1177/002215540305101213
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发表时间：
2003-12-01
影响因子：
3.2
通讯作者：
Nakayama, J
中科院分区：
文献类型：
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作者：
Nakajima, K;Ota, H;Nakayama, J
胃腺黏液细胞产生I - I - I类黏液蛋白，它也存在于沿胰胆道的布鲁纳氏腺和黏液腺中，也存在于向胃粘膜分化的化生和腺癌中。最近，我们发现III类粘蛋白具有GlcNAcalpha1—>4Galbeta—>R，由alpha1,4- n -乙酰氨基葡萄糖转移酶（alphaGnT）形成。检查粘蛋白表位的组织特异性表达有助于澄清细胞系分化和确定组织学标本中转移性癌的起源部位。食道、胃、结肠、肝脏、胰腺、肺、肾、前列腺、乳腺和唾液腺切除癌，以及唾液腺腺瘤、结肠腺瘤和胃、胰腺、结肠和乳腺淋巴结转移性腺癌，经福尔马林固定、石蜡包埋的组织切片，免疫染色MUC6、alpha4GnT和GlcNAcalpha1- bbb4galbeta ->R。这些基因在胃、胰腺和胆管的正常组织、化生组织和腺癌组织以及肺粘液细支气管肺泡癌中均有表达。表达alphaGnT的细胞一致表达GlcNAcalpha1—>4Galbeta—>R。MUC6仅在正常唾液腺、胰腺、精囊、肾小管、结肠腺瘤以及正常组织和前列腺、乳腺腺癌中表达。没有组织单独对alpha4GnT显示免疫反应性。转移癌和原发癌组织的免疫组化（IHC）谱相似。MUC6、alpha4GnT和GlcNAcalpha1—bbb4galbeta—>R的IHC谱可能与诊断相关。
Gastric gland mucous cells produce class I I I mucin, which is also found in Brunner's glands and mucous glands along the pancreaticobiliary tract, and in metaplasia and adenocarcinomas differentiating towards gastric mucosa. Recently, we showed that class III mucin possesses GlcNAcalpha1-->4Galbeta-->R, formed by alpha1,4-N-acetylglucosaminyltransferase (alphaGnT). Examining the tissue-specific expression of mucin epitopes is useful to clarify cell-lineage differentiation and to identify the site of origin of metastatic carcinomas in histological specimens. Formalin-fixed, paraffin-embedded tissue sections from esophagus, stomach, colon, liver, pancreas, lung, kidney, prostate, breast, and salivary gland resected for carcinoma, as well as salivary gland adenoma, colon adenoma, and metastatic adenocarcinoma of lymph nodes from stomach, pancreas, colon, and breast, were immunostained for MUC6, alpha4GnT, and GlcNAcalpha1-->4Galbeta-->R. These were all expressed in normal, metaplastic, and adenocarcinoma tissues of stomach, pancreas, and bile duct, and in pulmonary mucinous bronchioloalveolar carcinomas. Cells expressing alphaGnT uniformly expressed GlcNAcalpha1-->4Galbeta-->R. Only MUC6 was expressed in normal salivary glands, pancreas, seminal vesicles, renal tubules, and colon adenomas, and in normal tissue and adenocarcinomas of prostate and breast. No tissues showed immunoreactivity for alpha4GnT alone. Immunohistochemistry (IHC) profiles were similar for metastatic carcinomas and primary carcinoma tissues. The IHC profiles for MUC6, alpha4GnT, and GlcNAcalpha1-->4Galbeta-->R may be diagnostically relevant.