Efficacy of a Live Attenuated Escherichia coli O78: K80 Vaccine in Chickens and Turkeys
Efficacy of a Live Attenuated Escherichia coli O78: K80 Vaccine in Chickens and Turkeys
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DOI：
10.1637/10326-081512-reg.1
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发表时间：
2013-06-01
期刊：
影响因子：
1.4
通讯作者：
Karaca, K.
中科院分区：
文献类型：
--
作者：
La Ragione, R. M.;Woodward, M. J.;Karaca, K.
利用禽致病性大肠杆菌（APEC）O 78强毒株aroA基因突变，构建了APEC候选活疫苗。该突变体在菌落形态、运动性、悬浮生长、血凝、刚果红结合、HEp-2细胞粘附以及表面抗原1型菌毛和鞭毛的形成方面与亲本野生型菌株高度相似，尽管卷曲菌毛的产生略有减少。突变体证明无毒接种到1日龄雏鸡喷雾应用时，再次在饮用水中，在7天的年龄。用O 78 aroA突变体接种的鸡和火鸡在6周龄时受到强毒APEC菌株的攻击。
A candidate live vaccine for avian pathogenic Escherichia coli (APEC) was constructed from a virulent field APEC O78 strain by mutation of the aroA gene. The mutant was highly similar to the parent wild-type strain in respect of colony morphology, motility, growth in suspension, hemagglutination, Congo Red binding, HEp-2 cell adhesion, and the elaboration of surface antigens type 1 fimbriae and flagella, although production of curli fimbriae was reduced marginally. The mutant proved avirulent when inoculated into 1-day-old chicks by spray application and when presented again in the drinking water at 7 days of age. Chickens and turkeys vaccinated with an O78 aroA mutant were protected against a challenge at 6 wk of age by virulent APEC strains.