Antifungal susceptibility profiles and drug resistance mechanisms of clinical Candida duobushaemulonii isolates from China.
Antifungal susceptibility profiles and drug resistance mechanisms of clinical Candida duobushaemulonii isolates from China.
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来自中国的临床念珠菌Duobushaemulonii分离株的抗真菌敏感性和耐药性机制。
DOI：
10.3389/fmicb.2022.1001845
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发表时间：
2022
影响因子：
5.2
通讯作者：
Xu, Ying-Chun
中科院分区：
文献类型：
--
作者：
Chen, Xin-Fei;Zhang, Han;Jia, Xin-Miao;Cao, Jin;Li, Li;Hu, Xin-Lan;Li, Ning;Xiao, Yu-Ling;Xia, Fei;Ye, Li-Yan;Hu, Qing-Feng;Wu, Xiao-Li;Ning, Li-Ping;Hsueh, Po-Ren;Fan, Xin;Yu, Shu-Ying;Huang, Jing-Jing;Xie, Xiu-Li;Yang, Wen-Hang;Li, Ying-Xing;Zhang, Ge;Zhang, Jing-Jia;Duan, Si-Meng;Kang, Wei;Wang, Tong;Li, Jin;Xiao, Meng;Hou, Xin;Xu, Ying-Chun
关键词：
双重念珠菌（Candida duobushaemulonii），即II型念珠菌复合体，与耳念珠菌密切相关，能够引起人类的侵袭性和非侵袭性感染。11株C.采用基质辅助激光解吸电离飞行时间质谱（MALDI-TOF）、VITEK 2酵母菌鉴定卡（YST）和内转录间隔区（ITS）序列分析等方法对分离到的12株duobushaemulonii进行鉴定。C.全基因组测序duobushaemulonii进行基因分型。此外，C. duobushaemulonii菌株通过Sensititre YeastOne™和临床和实验室研究所（CLSI）肉汤微量稀释板测试抗真菌敏感性。三个C VITEK 2无法鉴定duobushaemulonii。所有11株分离株均对黄曲霉素B有较高的最低抑菌浓度（MIC），均大于2 μg/ml。1株分离株对5-氟胞嘧啶的MIC值较高，≥64 μg/ml。所有分离株均为三唑类和棘白菌素类野生型（WT）。FUR 1变异可能导致C. duobushaemulonii对5-氟胞嘧啶具有高MIC。多布氏假丝酵母菌主要感染免疫力低下的患者，其对阿替霉素B的耐药性可能对临床治疗构成挑战。
Candida duobushaemulonii, type II Candida haemulonii complex, is closely related to Candida auris and capable of causing invasive and non-invasive infections in humans. Eleven strains of C. duobushaemulonii were collected from China Hospital Invasive Fungal Surveillance Net (CHIF-NET) and identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF), VITEK 2 Yeast Identification Card (YST), and internal transcribed spacer (ITS) sequencing. Whole genome sequencing of C. duobushaemulonii was done to determine their genotypes. Furthermore, C. duobushaemulonii strains were tested by Sensititre YeastOne™ and Clinical and Laboratory Institute (CLSI) broth microdilution panel for antifungal susceptibility. Three C. duobushaemulonii could not be identified by VITEK 2. All 11 isolates had high minimum inhibitory concentrations (MICs) to amphotericin B more than 2 μg/ml. One isolate showed a high MIC value of ≥64 μg/ml to 5-flucytosine. All isolates were wild type (WT) for triazoles and echinocandins. FUR1 variation may result in C. duobushaemulonii with high MIC to 5-flucytosine. Candida duobushaemulonii mainly infects patients with weakened immunity, and the amphotericin B resistance of these isolates might represent a challenge to clinical treatment.
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