An Overview of the Development and Application of Surface Plasmon Resonance Based Immunosensors for Detection of Small Molecules
An Overview of the Development and Application of Surface Plasmon Resonance Based Immunosensors for Detection of Small Molecules
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DOI：
10.1166/sl.2008.525
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发表时间：
2008-12
期刊：
影响因子：
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通讯作者：
N. Miura;D. Shankaran;K. Gobi;Toshikazu Kawaguchi;Sook jin Kim
中科院分区：
文献类型：
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作者：
N. Miura;D. Shankaran;K. Gobi;Toshikazu Kawaguchi;Sook jin Kim
开发用于鉴定和定量小分子的高性能分析方法是提高人类生活质量和寿命的日益增长的全球性问题。基于表面等离子体共振的光学免疫分析是一种很有前途的分析工具，用于快速和实时分析各种分析基质中的化学和生物物质，具有增强的灵敏度和选择性。在这篇文章中，我们提供了一个国家的最先进的SPR免疫检测的生物医学，食品，国防和环境利益的小分子的概述，特别强调在我们的实验室的最新发展。SPR与间接竞争性抑制的原理以及其他信号增强系统（三明治几何形状和金纳米粒子）在实现更高的灵敏度的组合进行了讨论沿着使用便携式仪器多分析物和同时检测小分子。
Development of high performance analytical methods for identification and quantification of small molecules is an ever increasing global issue in the enhancement of the quality and longevity of human life. SPR based optical immunoassay is one of the promising analytical tools for rapid and real-time analysis of chemical and biological substances in various analytical matrices with enhanced sensitivity and selectivity. In this article, we provide an overview of the state-of-the-art SPR immunoassay for detection of small molecules of biomedical, food, defense and environmental interest with a special emphasis on the recent developments in our laboratory. The combination of SPR with the principle of indirect competitive inhibition as well as other signal enhancement systems (sandwich geometry and gold nanoparticle) in the achievement of greater sensitivity is discussed along with the use of portable instrumentations for multi-analyte and simultaneous detection of small molecules.