Identification and expression of C2H2 transcription factor genes in Carica papaya under abiotic and biotic stresses
Identification and expression of C2H2 transcription factor genes in Carica papaya under abiotic and biotic stresses
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非生物和生物胁迫下番木瓜C2H2转录因子基因的鉴定与表达
DOI：
10.1007/s11033-012-1542-y
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发表时间：
2012-06-01
影响因子：
2.8
通讯作者：
Pan, Lin-jie
中科院分区：
文献类型：
--
作者：
Jiang, Ling;Pan, Lin-jie
C2 H2蛋白属于转录因子超家族，在植物的防御反应和各种生理过程中发挥重要作用。本研究旨在筛选和鉴定番木瓜对非生物和生物胁迫的防御反应相关的C2 H2蛋白。收集了47，483个木瓜表达序列标签（EST）的数据。利用BioEdit软件预测了87个C2 H2蛋白的全长cDNA核苷酸序列。所有91 C2 H2蛋白进行比对，并使用DNAman构建系统发育树。通过定量逆转录-聚合酶链反应（qRT-PCR）分析盐胁迫条件下42 C2 H2的表达水平。茉莉酸甲酯处理使ZF（23.4）和ZF（30，912.1）分别快速上调18.6倍和21.7倍。ZF（1.3）、ZF（138.44）、ZF（94.49）、ZF（29.160）和ZF（20.206）在低温处理后均显著下调（p < 0.01）。精胺处理使ZF（23.4）、ZF（161.1）和ZF（30，912.1）上调，而ZF 1.3、ZF（158.1）、ZF（249.5）、ZF（138.44）、ZF（94.49）、ZF（29.160）和ZF（20.206）显著下调。SA处理后，ZF（23.4）被上调，而ZF（1.3）、ZF（249.5）、ZF（94.94）、ZF（29.160）、ZF（138.44）和ZF（20.206）被显著抑制。接种番木瓜环斑病毒后，ZF（23.4）和ZF（30，912.1）基因表达显著上调。通过将pBS-ZF（30，912.1）-GFP转基因融合到番木瓜原生质体中，将ZF（30，912.1）亚细胞定位于细胞核中。本研究结果表明，ZF（30，912.1）可能是一个重要的TF，介导的非生物和生物胁迫的反应在番木瓜。
C2H2 proteins belong to a group of transcription factors (TFs) existing as a superfamily that plays important roles in defense responses and various other physiological processes in plants. The present study aimed to screen for and identify C2H2 proteins associated with defense responses to abiotic and biotic stresses in Carica papaya L. Data were collected for 47,483 papaya-expressed sequence tags (ESTs). The full-length cDNA nucleotide sequences of 87 C2H2 proteins were predicated by BioEdit. All 91 C2H2 proteins were aligned, and a phylogenetic tree was constructed using DNAman. The expression levels of 42 C2H2 were analyzed under conditions of salt stress by quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR). Methyl jasmonate treatment rapidly upregulated ZF (23.4) and ZF (30,912.1) by 18.6- and 21.7-fold, respectively. ZF (1.3), ZF (138.44), ZF (94.49), ZF(29.160), and ZF (20.206) were found to be downregulated after low temperature treatment at very significant levels (p < 0.01). ZF (23.4), ZF (161.1), and ZF (30,912.1) were upregulated while ZF1.3, ZF (158.1), ZF (249.5), ZF (138.44), ZF (94.49), ZF (29.160), and ZF(20.206) were significantly downregulated by Spermine treatment. ZF (23.4) was upregulated while ZF (1.3), ZF (249.5), ZF(94.94), ZF (29.160), ZF (138.44), and ZF (20.206) were significantly repressed after SA treatment. ZF (23.4) and ZF (30,912.1) were significantly upregulated after sap inoculation with papaya ringspot virus pathogen. ZF (30,912.1) was subcellularly localized in the nucleus by a transgenic fusion of pBS-ZF (30,912.1)-GFP into the protoplast of papaya. The results of the present study showed that ZF (30,912.1) could be an important TF that mediates responses to abiotic and biotic stresses in papaya.