CAPITO-a web server-based analysis and plotting tool for circular dichroism data
CAPITO-a web server-based analysis and plotting tool for circular dichroism data
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DOI：
10.1093/bioinformatics/btt278
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发表时间：
2013-07-15
期刊：
影响因子：
5.8
通讯作者：
Goerlach, Matthias
中科院分区：
文献类型：
--
作者：
Wiedemann, Christoph;Bellstedt, Peter;Goerlach, Matthias
动机：圆二色（CD）光谱是研究蛋白质折叠和验证纯化蛋白质正确折叠的最通用的工具之一。在这里，我们的目标是提供一个易于访问，用户友好和平台无关的工具，能够分析几乎任何格式的多个CD数据集，并将结果作为高质量的图形输出返回给用户。结果：CAPITO （CD analysis and绘图工具）是一种基于web服务器的CD数据分析和绘图工具。它允许使用不同的方法对二级结构内容进行可靠的估计。CAPITO接受多个CD数据集，因此非常适合广泛的应用范围，例如分析温度或ph依赖（非）折叠以及突变体的比较。
Motivation: Circular dichroism (CD) spectroscopy is one of the most versatile tools to study protein folding and to validate the proper fold of purified proteins. Here, we aim to provide a readily accessible, userfriendly and platform-independent tool capable of analysing multiple CD datasets of virtually any format and returning results as high-quality graphical output to the user.Results: CAPITO (CD Anaylsis and Plotting Tool) is a novel web server-based tool for analysing and plotting CD data. It allows reliable estimation of secondary structure content utilizing different approaches. CAPITO accepts multiple CD datasets and, hence, is well suited for a wide application range such as the analysis of temperature or pH-dependent (un)folding and the comparison of mutants.