Primary concentration - The critical step in implementing the wastewater based epidemiology for the COVID-19 pandemic: A mini-review
Primary concentration - The critical step in implementing the wastewater based epidemiology for the COVID-19 pandemic: A mini-review
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DOI：
10.1016/j.scitotenv.2020.141245
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发表时间：
2020-12-10
影响因子：
9.8
通讯作者：
Sha, Sha
中科院分区：
文献类型：
--
作者：
Lu, Dingnan;Huang, Zhuangrong;Sha, Sha
最近爆发的新型冠状病毒SARS-CoV-2对全球公共卫生构成了重大威胁，并造成了严重的社会和经济破坏。一个新的研究方向是吸引了大量的关注，在学术界的环境科学和工程，其中快速社区水平的监测可以实现通过应用基于废水的流行病学（WBE）的方法。鉴于废水样品和感染粪便样品中病毒RNA拷贝总数的积累平衡的发展是WBE的核心，废水中定量RNA检测的结果必须高度灵敏、准确和可靠。因此，在随后的RNA提取和RT-qPCR检测之前应用有效的浓缩方法是WBE的必备程序。本综述提供了对最近报道的18项COVID-19废水检测研究所采用的主要浓缩方法的新见解，沿着简要讨论了最常用的病毒浓缩方法的机制，包括基于PEG的分离，静电荷膜过滤和超滤。最后，提出了两种简单可行的浓缩策略：预过滤-加盐-电负性膜过滤（初始体积为50 mL）。策略2：预过滤-基于PEG的分离-静置过夜（初始体积为50 - 1000 mL）。(C)2020爱思唯尔B.V.保留所有权利。
The recent outbreak of a novel coronavirus SARS-CoV-2 has posed a significant global public health threat and caused dramatic social and economic disruptions. A new research direction is attracting a significant amount of attention in the academic community of environmental sciences and engineering, in which rapid community-levelmonitoring could be achieved by applying the methodology of wastewater based epidemiology (WBE). Given the fact that the development of amass balance on the total number of viral RNA copies in wastewater samples and the infected stool specimens is the heart of WBE, the result of the quantitative RNA detection in wastewater has to be highly sensitive, accurate, and reliable. Thus, applying effective concentration methods before the subsequent RNA extraction and RT-qPCR detection is a must-have procedure for the WBE. This review provides new insights into the primary concentration methods that have been adopted by the eighteen recently reported COVID-19 wastewater detection studies, along with a brief discussion of the mechanisms of the most commonly used virus concentration methods, including the PEG-based separation, electrostatically charged membrane filtration, and ultrafiltration. In the end, two easy and well-proven concentration strategies are recommended as below, aiming to maximize the practical significance and operational effectiveness of the SARS-CoV-2 virus concentration from wastewater samples.Strategy1: Prefiltration-Salt addition-Electronegative membrane filtration (for initial volume = 50 mL). Strategy2: Prefiltration-PEG-based separation-Overnight standing (for initial volume from 50 to 1000 mL). (C) 2020 Elsevier B.V. All rights reserved.