The excess microsporocytes1 gene encodes a putative leucine-rich repeat receptor protein kinase that controls somatic and reproductive cell fates in the Arabidopsis anther.
The excess microsporocytes1 gene encodes a putative leucine-rich repeat receptor protein kinase that controls somatic and reproductive cell fates in the Arabidopsis anther.
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DOI：
10.1101/gad.997902
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发表时间：
2002-08
影响因子：
10.5
通讯作者：
Dazhong Zhao;Guanfang Wang;Brooke Speal;Hong Ma
中科院分区：
文献类型：
--
作者：
Dazhong Zhao;Guanfang Wang;Brooke Speal;Hong Ma
细胞分化对多细胞生物体的发育至关重要。在开花植物中，单倍体的雄配子体(花粉粒)是由称为小孢子母细胞的生殖细胞在花药中产生的。几种类型的体细胞保证了花粉的成功发育，从而保证了繁殖。然而，目前还不清楚是什么基因控制着这些花药中这些多样化、高度特化的细胞的分化。本文报道了一种新的拟南芥雄性不育突变体--过量小孢子球1(Ems1)的分离和鉴定，该突变体产生过量的小孢子细胞，缺乏绒毛细胞，并异常地维持中层细胞。虽然ems1突变体的减数分裂核分裂正常，但小孢子母细胞没有进行胞质分裂，导致小孢子发生失败和雄性不育。EMS1基因编码一个可能的富含亮氨酸重复序列的受体蛋白激酶(LRR-RPK)，其表达与小孢子母细胞和绒毛细胞的分化有关，表明EMS1介导了控制生殖细胞及其毗邻的体细胞命运的信号。
Cell differentiation is essential for the development of multicellular organisms. In flowering plants, the haploid male gametophytes (pollen grains) are generated in the anther from reproductive cells called microsporocytes. Several types of somatic cells ensure successful pollen development, and thus reproduction. However, it is not clear what genes regulate the differentiation of these diverse, highly specialized cells in the anther. We report here the isolation and characterization of a novel Arabidopsis thaliana male sterile mutant, excess microsporocytes1 (ems1), that produces excess microsporocytes, lacks tapetal cells, and abnormally maintains middle layer cells. Although the meiotic nuclear division in the ems1 mutant is normal, the microsporocytes do not undergo cytokinesis, resulting in failed microsporogenesis and male sterility. The EMS1 gene encodes a putative leucine-rich repeat receptor protein kinase (LRR-RPK), and its expression is associated with the differentiation of the microsporocytes and tapetal cells, suggesting that EMS1 mediates signals that control the fate of reproductive cells and their contiguous somatic cells.