Calorimetry Methods to Study Membrane Interactions and Perturbations Induced by Antimicrobial Host Defense Peptides
Calorimetry Methods to Study Membrane Interactions and Perturbations Induced by Antimicrobial Host Defense Peptides
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DOI：
10.1007/978-1-4939-6737-7_9
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发表时间：
2017-01-01
期刊：
影响因子：
--
通讯作者：
Vogel, Hans J.
中科院分区：
文献类型：
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作者：
Arias, Mauricio;Prenner, Elmar J.;Vogel, Hans J.
生物膜在确定抗菌宿主防御肽（AMP）的活性和选择性方面发挥着重要作用。已经开发了几种生物物理方法来研究 AMP 与生物膜的相互作用。等温滴定量热法和差示扫描量热法（分别为 ITC 和 DSC）是强大的技术，因为它们提供了独特的无标记方法。 ITC 可以对 AMP 和膜之间的相互作用进行完整的热力学表征。 DSC 允许人们研究肽结合对膜中磷脂堆积的影响。与生物膜的模拟模型（例如磷脂囊泡）结合使用，可以表征不同磷脂头基和不同酰基链的作用。在这些协议中，将介绍使用 ITC 和 DSC 方法研究肽-膜相互作用，强调选择代表细菌和哺乳动物细胞的膜模型系统的重要性。这些研究为 AMP 的膜结合和扰动特性所涉及的机制提供了有价值的见解。
Biological membranes play an important role in determining the activity and selectivity of antimicrobial host defense peptides (AMPs). Several biophysical methods have been developed to study the interactions of AMPs with biological membranes. Isothermal titration calorimetry and differential scanning calorimetry (ITC and DSC, respectively) are powerful techniques as they provide a unique label-free approach. ITC allows for a complete thermodynamic characterization of the interactions between AMPs and membranes. DSC allows one to study the effects of peptide binding on the packing of the phospholipids in the membrane. Used in combination with mimetic models of biological membranes, such as phospholipid vesicles, the role of different phospholipid headgroups and distinct acyl chains can be characterized. In these protocols the use of ITC and DSC methods for the study of peptide-membrane interactions will be presented, highlighting the importance of membrane model systems selected to represent bacterial and mammalian cells. These studies provide valuable insights into the mechanisms involved in the membrane binding and perturbation properties of AMPs.