Nucleotide sequence of the thymidine kinase gene of herpes simplex virus type 1.
Nucleotide sequence of the thymidine kinase gene of herpes simplex virus type 1.
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1型单纯疱疹病毒胸苷激酶基因的核苷酸序列。
DOI：
10.1073/pnas.78.3.1441
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发表时间：
1981
影响因子：
11.1
通讯作者：
Summers,WC
中科院分区：
文献类型：
--
作者：
Wagner,MJ;Sharp,JA;Summers,WC
我们已经从Enquist等人[Enquist，L.]获得的病毒DNA的质粒克隆中确定了单纯疱疹病毒1型CL 101株的胸苷激酶（ATP：胸苷5 ′磷酸转移酶，EC2.7.1.21）基因的完整核苷酸序列。W.，Vande Woude，G. F.、瓦格纳，M.，斯迈利，J.R. & Summers，W. C.（1979）Gene 7，335-342]。还分析了胸苷激酶mRNA的5'端的cDNA拷贝以定位转录序列。该基因的转录部分长度约为1300个核苷酸，似乎不含间插序列。在mRNA的5'端有一个107个核苷酸的非翻译区，随后是一个1128个核苷酸的开放阅读框。因此，该基因能够编码376个氨基酸的蛋白质。疱疹病毒胸苷激酶基因中也存在与被认为参与控制多种真核和病毒基因的转录和翻译的序列类似的序列，例如“Hogness盒”和A-A-T-A-A-A多聚腺苷酸化信号。
We have determined the complete nucleotide sequence of the thymidine kinase (ATP:thymidine 5' phosphotransferase, EC 2.7.1.21) gene of herpes simplex virus type 1 strain CL101 from a plasmid clone of viral DNA derived by Enquist et al. [Enquist, L. W., Vande Woude, G. F., Wagner, M., Smiley, J. R. & Summers, W. C. (1979) Gene 7, 335-342]. A cDNA copy of the 5' end of thymidine kinase mRNA was also analyzed to locate the transcribed sequences. The transcribed portion of the gene is approximately 1300 nucleotides in length and appears to contain no intervening sequences. There is an untranslated region of 107 nucleotides at the 5' end of the mRNA followed by an open reading frame of 1128 nucleotides. The gene is thus capable of coding for a protein of 376 amino acids. Sequences similar to those thought to be involved in control of transcription and translation of a variety of eukaryotic and viral genes such as a "Hogness box" and A-A-T-A-A-A polyadenylylation signals are also present in the herpesvirus thymidine kinase gene.