Surface modified electrospun nanofibrous scaffolds for nerve tissue engineering
Surface modified electrospun nanofibrous scaffolds for nerve tissue engineering
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DOI：
10.1088/0957-4484/19/45/455102
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发表时间：
2008-11-12
期刊：
影响因子：
3.5
通讯作者：
Ramakrishna, S.
中科院分区：
文献类型：
--
作者：
Prabhakaran, Molamma P.;Venugopal, J.;Ramakrishna, S.
生物可降解高分子支架的表面特性决定了材料与生物环境之间的相互作用，其开发在生物医学应用中具有重要意义。在这方面，通过静电纺丝工艺制备聚epsilon-己内酯（PCL）纳米纤维支架，并通过简单的等离子体处理工艺对其表面进行修饰，以增强雪旺细胞的粘附、增殖以及与神经组织形成所必需的纳米纤维的相互作用。通过接触角和x射线光电子能谱研究了表面改性PCL纳米纤维支架（p-PCL）的亲水性。天然来源的聚合物，如胶原蛋白，经常用于制造生物复合材料PCL/胶原蛋白支架，尽管为临床应用采购大量天然材料的可行性，以及它们的成本和机械稳定性仍然是一个问题。细胞培养8天后，p-PCL纳米纤维支架上的雪旺细胞增殖能力比PCL/胶原纳米纤维支架上的雪旺细胞增殖能力提高17%。发现雪旺细胞在表达双极性延长的表面修饰的PCL纳米纤维支架上附着和增殖，保持其正常形态。我们的研究结果表明，与PCL/胶原支架相比，等离子体处理的PCL纳米纤维支架是一种具有成本效益的材料，可以作为理想的组织工程支架，特别是周围神经再生。
The development of biodegradable polymeric scaffolds with surface properties that dominate interactions between the material and biological environment is of great interest in biomedical applications. In this regard, poly-epsilon-caprolactone (PCL) nanofibrous scaffolds were fabricated by an electrospinning process and surface modified by a simple plasma treatment process for enhancing the Schwann cell adhesion, proliferation and interactions with nanofibers necessary for nerve tissue formation. The hydrophilicity of surface modified PCL nanofibrous scaffolds (p-PCL) was evaluated by contact angle and x-ray photoelectron spectroscopy studies. Naturally derived polymers such as collagen are frequently used for the fabrication of biocomposite PCL/collagen scaffolds, though the feasibility of procuring large amounts of natural materials for clinical applications remains a concern, along with their cost and mechanical stability. The proliferation of Schwann cells on p-PCL nanofibrous scaffolds showed a 17% increase in cell proliferation compared to those on PCL/collagen nanofibrous scaffolds after 8 days of cell culture. Schwann cells were found to attach and proliferate on surface modified PCL nanofibrous scaffolds expressing bipolar elongations, retaining their normal morphology. The results of our study showed that plasma treated PCL nanofibrous scaffolds are a cost-effective material compared to PCL/collagen scaffolds, and can potentially serve as an ideal tissue engineered scaffold, especially for peripheral nerve regeneration.