Production of angiotensinogen and renin-like activity by rabbit proximal tubular cells in culture.
Production of angiotensinogen and renin-like activity by rabbit proximal tubular cells in culture.
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培养的兔近端肾小管细胞产生血管紧张素原和肾素样活性。
DOI：
10.1038/ki.1991.117
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发表时间：
1991
影响因子：
19.6
通讯作者：
Eggena,P
中科院分区：
文献类型：
--
作者：
Yanagawa,N;Capparelli,AW;Jo,OD;Friedal,A;Barrett,JD;Eggena,P
培养的兔近端肾小管细胞产生血管紧张素原和肾素样活性。最近的研究表明肾脏中存在局部血管紧张素生成系统。通过原位杂交技术，血管紧张素原的信使核糖核酸已被证明存在于近端小管中。在本研究中，我们试图检测近曲(PCT)和直曲(PST)肾小管细胞产生血管紧张素原和肾素样活性。从显微解剖的兔近端小管中分离出PCT和PST细胞，并进行体外培养。对培养上清液和细胞裂解液中的血管紧张素原和肾素样活性进行定量。结果表明，PCT培养液中同时含有血管紧张素原和肾素样活性，而PST培养液中仅检测到血管紧张素原。PCT培养液中的血管紧张素原和肾素样活性在特定的无血清培养液中以时间依赖和激素敏感的方式增加，这为从头合成提供了支持。这些结果证实了近端肾小管上皮细胞确实产生了血管紧张素原和肾素样活性，并提示这些局部合成的成分可能参与了肾近端小管血管紧张素生成的调节。
Production of angiotensinogen and renin-like activity by rabbit proximal tubular cells in culture. Recent studies suggest the presence of local angiotensin generating system in the kidney. By using in situ hybridization technique, mRNA for angiotensinogen has been shown to be present in the proximal tubule. In the present study, we have attempted to examine the production of angiotensinogen and renin-like activity by the proximal convoluted (PCT) and straight (PST) tubular cells. PCT and PST cells were obtained from microdissected rabbit proximal tubules and cultured in vitro. Angiotensinogen and renin-like activity were quantitated in culture media and cell lysates. It was found that PCT culture medium contained both angiotensinogen and renin-like activity, whereas only angiotensinogen was detected in PST culture medium. Support for de novo synthesis is provided by the observation that both angiotensinogen and renin-like activity in PCT culture medium increased in a time-dependent and hormone-sensitive manner in defined serum-free medium. These results thus demonstrate the actual production of angiotensinogen and renin-like activity by proximal tubular cells, and indicate that these locally synthesized components may contribute to the regulation of angiotensin generation in renal proximal tubule.