Antibiotic resistance genes, class 1 integrons, and IncP-1/IncQ-1 plasmids in irrigation return flows
Antibiotic resistance genes, class 1 integrons, and IncP-1/IncQ-1 plasmids in irrigation return flows
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DOI：
10.1016/j.envpol.2019.113568
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发表时间：
2020-02-01
影响因子：
8.9
通讯作者：
Bjorneberg, David L.
中科院分区：
文献类型：
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作者：
Dungan, Robert S.;Bjorneberg, David L.
地表沃茨可能是抗生素耐药基因传播的主要途径。在本研究中，我们探讨了在爱达荷州南部的上蛇岩流域的8个灌溉回流（IRF）和一个背景点（Main Line Canal，MLC）中ARG [bla（CTX-M-1）、ARG（B）、sul 1、泰特（B）、泰特（M）和泰特（X）]、1类整合子整合酶基因（intI 1）以及IncP-1和IncQ-1质粒的流行率和丰度。在一个日历年内每月采集抓取样本，对其进行处理以提取微生物DNA，然后进行液滴数字PCR以在绝对（每100 mL）和相对（每16 S rRNA基因拷贝）基础上定量基因拷贝。在所有IRF采样点都发现了抗生素耐药性和intI 1基因以及IncP-1/IncQ-1质粒，在81个水样采样事件中检测到55至81个。bla（CTX-M-1）基因的检出率最低（68%），而其他基因的检出率较高（88-100%）。在4月至10月的MLC中，当运河中存在水时，也检测到所有的基因。IRFs中相对丰度从低到高的基因为：bla（CTX-M-1）<IncP-1 < tet（B）< tet（B）< IncQ-1 <泰特（M）<sul 1 <intI 1 = IncP-1 <泰特（X）。当与MLC水样中的年均相对基因丰度相比时，发现它们处于统计学上更高的水平（P
Surface waters could be a dominant route by which antibiotic resistance genes (ARGs) are disseminated. In the present study we explored the prevalence and abundance of ARGs [bla(CTX-M-1), erm(B), sul1, tet(B), tet(M), and tet(X)], class 1 integron-integrase gene (intI1), and IncP-1 and IncQ-1 plasmids in eight irrigation return flows (IRFs) and a background site (Main Line Canal, MLC) in the Upper Snake Rock watershed in southern Idaho. Grab samples were collected on a monthly basis for a calendar year, which were processed to extract microbial DNA, followed by droplet digital PCR to quantify the gene copies on an absolute (per 100 mL) and relative (per 16S rRNA gene copies) basis. The antibiotic resistance and intI1 genes and IncP-1/IncQ-1 plasmids were recovered at all IRF sampling sites with detections ranging from 55 to 81 out of 81 water sampling events. The bla(CTX-M-1) gene was detected the least frequently (68%), while the other genes were detected more frequently (88-100%). All of the genes were also detected at MLC from April to Oct when water was present in the canal. The genes from lowest to greatest relative abundance in the IRFs were: bla(CTX-M-1) < erm(B) < tet(B) < IncQ-1 < tet(M) < sul1 < intI1 = IncP-1 < tet(X). When compared to the average annual relative gene abundances in MLC water samples, they were found to be at statistically greater levels (P