Synchrony induced by double phosphate starvation in a suspension culture of Catharanthus roseus
Synchrony induced by double phosphate starvation in a suspension culture of Catharanthus roseus
复制标题
长春花悬浮培养物中双磷酸盐饥饿诱导的同步性
DOI：
10.1111/j.1399-3054.1983.tb04220.x
复制
发表时间：
1983
影响因子：
6.4
通讯作者：
A. Komamine
中科院分区：
文献类型：
--
作者：
S. Amino;T. Fujimura;A. Komamine
利用双磷酸盐饥饿法建立了长春花悬浮培养的细胞同步分裂体系。在Murashige和Skoog的培养液中，Don细胞为磷酸盐。在该系统中，细胞数量仅在短短4h内增加，而在细胞周期的其他阶段，细胞数量几乎保持不变。有丝分裂指数的变化证实了培养的同步性，在细胞数量增加之前，有丝分裂指数急剧增加。通过测定细胞周期中[~3H]-胸腺嘧啶核苷的掺入，确定细胞的S时相，同时也验证了DNA合成的同步性。磷酸盐饥饿引起的同步化与磷酸盐作为营养物质的功能有关。这样建立的同步系统将有助于高等植物细胞周期的生化研究。
A synchronous cell division system was established using the double phosphate starvation method, based on the observation that one of the limiting factors in the growth of a suspension culture of Catharanthus roseus (L.) G. Don cells in the medium of Murashige and Skoog was phosphate. In the system, an increase in cell number took place in a short period of only 4 h, while the cell number remained almost constant during other periods of the cell cycle. The synchrony of the culture was confirmed by changes in mitotic index, which increased sharply prior to the increase in cell number. The S phase was determined by measuring incorporation of [3H]-thymidine into the DNA fraction during the cell cycle and synchrony of DNA synthesis was verified likewise. Synchronization by phosphate starvation is discussed in relation to the function of phosphate as a nutrient. The synchronous system thus established will be useful in biochemical studies of the cell cycle in higher plants.