Alteration of the timing of implantation by in vivo gene transfer:: delay of implantation by suppression of nuclear factor κB activity and partial rescue by leukemia inhibitory factor
Alteration of the timing of implantation by in vivo gene transfer:: delay of implantation by suppression of nuclear factor κB activity and partial rescue by leukemia inhibitory factor
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DOI：
10.1016/j.bbrc.2004.07.045
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发表时间：
2004-09-03
影响因子：
3.1
通讯作者：
Murata, Y
中科院分区：
文献类型：
--
作者：
Nakamura, H;Kimura, T;Murata, Y
核因子kappaB （NF-kappaB）在着床期小鼠子宫内膜中的激活[j]。j .天线转换开关。免疫学杂志，51(2004)16]。用日本血凝病毒包膜载体将IkappaBalpha突变体（IkappaBalphaM） cDNA瞬时转染小鼠子宫腔，在第3.5天和4.5天对子宫NF-kappaB活性的抑制不到对照组的一半，转染IkappaBalphaM cDNA可显著延迟着床时间。转染IkappaBalphaM cDNA后，子宫内LIF mRNA的表达在第3.5天和第4.5天明显受到抑制。在子宫内，LIF cDNA与IkappaBalphaM cDNA共同转染，部分挽救了NF-kappaB活性抑制引起的着床延迟。综上所述，这些发现表明NF-kappaB的激活至少在一定程度上通过对LIF表达的控制来决定着床时间。(C) 2004爱思唯尔公司版权所有。
Nuclear factor kappaB (NF-kappaB) is activated in the murine endometrium during implantation period [Am. J. Reprod. Immunol. 51 (2004) 16]. Transient transfection of IkappaBalpha mutant (IkappaBalphaM) cDNA into the mouse uterine cavity using hemagglutinating virus of Japan envelope vector suppressed uterine NF-kappaB activity less than half of that observed in control on days 3.5 and 4.5 p.c. IkappaBalphaM cDNA transfection led to significant delay of implantation. After IkappaBalphaM cDNA transfection, LIF mRNA expression in the uterus was significantly suppressed on days 3.5 and 4.5 p.c. Co-transfection of LIF cDNA with IkappaBalphaM cDNA in the uterus partially rescued the delay of implantation induced by suppression of NF-kappaB activity. Taken together, these findings indicate that NF-kappaB activation determines the timing of the implantation, at least in part, via control of LIF expression. (C) 2004 Elsevier Inc. All rights reserved.