Ephrin-B stimulation of calvarial bone formation
Ephrin-B stimulation of calvarial bone formation
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DOI：
10.1002/dvdy.23874
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发表时间：
2012-12-01
影响因子：
2.5
通讯作者：
Chenaux, George
中科院分区：
文献类型：
--
作者：
Benson, M. Douglas;Opperman, Lynne A.;Chenaux, George
简介：据报道，破骨细胞上的Ephrin-B2通过激活成骨细胞上的EphB 4酪氨酸激酶受体来促进骨形成，作为体内平衡的一部分。关于肝配蛋白-B信号传导至EphBs在发育性骨形成中的作用知之甚少。结果如下：我们观察到胚胎和新生小鼠的骨膜、骨缝和硬脑膜中肝配蛋白-B2 LacZ嵌合等位基因的表达。在成人颅骨中的表达仅限于缝合处，但在骨损伤部位严重上调。用可溶性重组肝配蛋白-B2/Fc培养胚胎颅骨使其骨含量加倍，而不改变骨缝宽度或整体颅骨形态。Ephrin-B2/Fc还刺激了培养的MC 3 T3前成骨细胞中的成骨细胞标志物基因表达，而不需要1型胶原诱导的分化。EphB 4在胚胎和成人颅骨中不存在。然而，EphB 1和EphB 2，肝配蛋白-Bs的生理受体，表达在骨生成的网站，和EphB 1敲除小鼠显示出减少颅骨骨含量相比，对照组。结论：这些数据支持ephrin-B2通过激活成骨细胞特异性基因表达在骨发育和愈合中的作用。EphB 1和EphB 2可能是骨中ephrin-B2的候选受体。发展动力学，2012年。(C)2012年威利期刊公司
Introduction: Ephrin-B2 on osteoclasts was reported to promote bone formation as part of homeostasis by activating the EphB4 tyrosine kinase receptor on osteoblasts. Little is known about the role of ephrin-B signaling to EphBs in developmental bone formation. Results: We observed expression of an ephrin-B2 LacZ chimeric allele in the periosteum, sutural bone fronts, and dura mater of embryonic and neonatal mice. Expression in the adult skull was confined to sutures, but was heavily upregulated at sites of bone injury. Culture of embryonic calvariae with soluble recombinant ephrin-B2/Fc doubled their bone content without altering suture width or overall skull morphology. Ephrin-B2/Fc also stimulated osteoblast marker gene expression in cultured MC3T3 preosteoblastic cells without the need for type 1 collagen-induced differentiation. EphB4 was absent in embryonic and adult skulls. However, EphB1 and EphB2, both physiological receptors for ephrin-Bs, were expressed at sites of osteogenesis, and EphB1 knockout mice displayed a reduction in calvarial bone content compared to controls. Conclusions: These data support a role for ephrin-B2 in the development and healing of bone through activation of osteoblast-specific gene expression. EphB1 and EphB2 are likely candidates receptors for the ephrin-B2 in bone. Developmental Dynamics, 2012. (C) 2012 Wiley Periodicals, Inc.