Expression profiling of H3K27me3 demethylase genes during plant development and in response to environmental stress in Arabidopsis
Expression profiling of H3K27me3 demethylase genes during plant development and in response to environmental stress in Arabidopsis
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DOI：
10.1080/15592324.2021.1950445
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发表时间：
2021-07
影响因子：
2.9
通讯作者：
Nobutoshi Yamaguchi;Toshiro Ito
中科院分区：
文献类型：
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作者：
Nobutoshi Yamaguchi;Toshiro Ito
组蛋白修饰影响基因表达。在组蛋白修饰中，H3K27me3通过染色质压缩与附近基因的下调相关。在拟南芥（Arabidopsis thaliana）中，JMJ蛋白的一个子集在植物发育过程中或响应环境信号时去除H3K27me3中起关键作用。然而，H3K27me3去甲基化酶基因表达的调节尚未完全表征。在这项研究中，我们使用在植物发育过程中和各种环境线索后创建的公共转录组数据集，计算表征了JMJ H3 K27 me3脱甲基酶基因的表达模式。与可用的转录组数据集一致，GUS染色验证了JMJ 30在茎尖分生组织的L1层中高度表达。此外，5个H3K27me3脱甲基酶基因的表达数据显示JMJ 30受非生物和生物胁迫的影响最大。此外，JMJ 30的表达是可变的拟南芥之间加入。最后，表达的JMJ 30直系同源物从相关物种拟南芥，AhgJMJ 30，波动在田间条件下。综上所述，我们的研究结果表明，H3K27me3去甲基化酶基因的转录变化可能在发育和环境响应中发挥关键作用。
ABSTRACT Histone modification influences gene expression. Among histone modifications, H3K27me3 is associated with downregulation of nearby genes via chromatin compaction. In Arabidopsis thaliana, a subset of JUMONJI C DOMAIN-CONTAINING PROTEIN (JMJ) proteins play a critical role in removal of H3K27me3 during plant development or in response to environmental cues. However, the regulation of H3K27me3 demethylase gene expression is not yet fully characterized. In this study, we computationally characterized the expression patterns of JMJ H3K27me3 demethylase genes using public transcriptome datasets created across plant development and after various environmental cues. Consistent with the available transcriptome datasets, GUS staining validated that JMJ30 was highly expressed in the L1 layer of the shoot apical meristem. Furthermore, expression data for panel of five H3K27me3 demethylase genes revealed JMJ30 to be the most highly affected by abiotic and biotic stress. In addition, JMJ30 expression was variable between Arabidopsis thaliana accessions. Finally, the expression of a JMJ30 orthologue from the related species Arabidopsis halleri, AhgJMJ30, fluctuated under field conditions. Taken together, our results suggest that transcriptional changes of H3K27me3 demethylase genes may play key roles in development and environmental responses.