Detection and clinical significance of circulating tumor cells in colorectal cancer.
Detection and clinical significance of circulating tumor cells in colorectal cancer.
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DOI：
10.1186/s40364-021-00326-4
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发表时间：
2021-11-19
影响因子：
11.1
通讯作者：
Zong H
中科院分区：
文献类型：
--
作者：
Jiang M;Jin S;Han J;Li T;Shi J;Zhong Q;Li W;Tang W;Huang Q;Zong H
组织病理学检查（活检）是诊断结直肠癌（CRC）的“金标准”。然而，活检是一种侵入性的方法，由于肿瘤的时空异质性，单次活检不能全面揭示肿瘤的生物学特征和动态变化。因此，需要新的生物标志物来提高结直肠癌的诊断和对结直肠癌患者的监测和治疗。大量研究表明，“液体活检”是一种很有前途的早期CRC微创检测方法。液体活检主要采集循环肿瘤细胞（CTCs）、循环肿瘤DNA （ctDNA）、microRNA （miRNA）和细胞外囊泡（ev）。ctc是从原发肿瘤脱落和/或转移到外周循环的恶性细胞。CTCs携带原发肿瘤和转移肿瘤的信息，能够及时反映肿瘤的动态变化。ctc是一种很有前景的生物标志物，可用于疾病的早期检测、治疗反应和疾病进展评估、疾病机制阐明、药物开发的治疗靶点鉴定。本文就目前血浆CTC的分离和检测技术、在结直肠癌患者管理中的应用及未来的研究方向进行综述。
Histopathological examination (biopsy) is the “gold standard” for the diagnosis of colorectal cancer (CRC). However, biopsy is an invasive method, and due to the temporal and spatial heterogeneity of the tumor, a single biopsy cannot reveal the comprehensive biological characteristics and dynamic changes of the tumor. Therefore, there is a need for new biomarkers to improve CRC diagnosis and to monitor and treat CRC patients. Numerous studies have shown that “liquid biopsy” is a promising minimally invasive method for early CRC detection. A liquid biopsy mainly samples circulating tumor cells (CTCs), circulating tumor DNA (ctDNA), microRNA (miRNA) and extracellular vesicles (EVs). CTCs are malignant cells that are shed from the primary tumors and/or metastases into the peripheral circulation. CTCs carry information on both primary tumors and metastases that can reflect dynamic changes in tumors in a timely manner. As a promising biomarker, CTCs can be used for early disease detection, treatment response and disease progression evaluation, disease mechanism elucidation, and therapeutic target identification for drug development. This review will discuss currently available technologies for plasma CTC isolation and detection, their utility in the management of CRC patients and future research directions.
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影响因子：
12.6
作者：
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通讯作者：
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影响因子：
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作者：
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影响因子：
3.8
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Salanti A
影响因子：
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作者：
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通讯作者：
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