An improved simple method of mouse lung intubation
An improved simple method of mouse lung intubation
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DOI：
10.1152/japplphysiol.91376.2008
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发表时间：
2009-03-01
影响因子：
3.3
通讯作者：
Mitzner, Wayne
中科院分区：
文献类型：
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作者：
MacDonald, Kelvin D.;Chang, Herng-Yu Sucie;Mitzner, Wayne
放大图片作者：Michael W.一种改进的小鼠肺插管简易方法。J Appl Physiol 106：984-987，2009.首次出版于2009年1月15日。鉴于小鼠广泛用于研究肺部疾病，奇怪的是，更多的研究人员没有使用重复插管来研究单个小鼠的机械和细胞变化。这种有限的使用插管的原因之一是，它是相对困难的，尽管有几个已发表的研究，描述的方法来实现it. In本文中，我们描述了一个完整的程序，包括新的方法，简化这种插管，使它可以常规完成相对较少的培训。这项技术也可以建立在相对较少的费用和专业知识。这将使任何实验室都可以常规进行插管，从而允许对单个小鼠进行纵向研究，并通过使用每只小鼠作为其自身对照来增加统计功效。
MacDonald KD, Chang HY, Mitzner W. An improved simple method of mouse lung intubation. J Appl Physiol 106: 984-987, 2009. First published January 15, 2009;.-Given the ubiquitous use of mice to study lung disease, it is curious that more investigators do not use repeated intubation to study mechanical and cellular changes in individual mice. One of the reasons for this limited use of intubation is that it is relatively difficult, despite there being several published studies that describe ways to achieve it. In this paper, we describe a complete procedure, including novel approaches that simplify this intubation, so that it can be routinely accomplished with relatively little training. The technique can also be set up with relatively little expense and expertise. This should make it possible for any laboratory to routinely carry out this intubation, thereby allowing longitudinal studies in individual mice and potentially increasing the statistical power by using each mouse as its own control.