The combination of db-cAMP and ChABC with poly(propylene carbonate) microfibers promote axonal regenerative sprouting and functional recovery after spinal cord hemisection injury
The combination of db-cAMP and ChABC with poly(propylene carbonate) microfibers promote axonal regenerative sprouting and functional recovery after spinal cord hemisection injury
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db-cAMP和ChABC与聚碳酸亚丙酯微纤维的组合促进脊髓半切损伤后轴突再生萌芽和功能恢复
DOI：
10.1016/j.biopha.2016.12.045
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发表时间：
2017
影响因子：
7.5
通讯作者：
Li Xingang
中科院分区：
文献类型：
--
作者：
Xia Tongliang;Huang Bin;Ni Shilei;Gao Lei;Wang Jiangang;Wang Jian;Chen Anjing;Zhu Shaowei;Wang Benlin;Li Gang;Zhu Shugan;Li Xingang
本研究描述了使用聚（碳酸亚丙酯）（PPC）的电纺微纤维浸渍与二丁酰环磷酸腺苷（db-cAMP）和软骨素酶ABC（ChABC）的组合在治疗右侧半切脊髓损伤（SCI）。释放的db-cAMP和/或ChABC从微纤维进行了评估，在体外使用高效液相色谱法（HPLC）。将药物浸渍的微纤维植入大鼠的半切胸脊髓中，并使用功能恢复检查和免疫组织化学评价治疗。我们的结果表明，含有db-cAMP和/或ChABC的微纤维显示出每种药剂的稳定和延长的释放。发现持续递送db-cAMP和/或ChABC与未处理的对照动物相比促进轴突再生发芽、功能恢复和减少神经胶质瘢痕形成。db-cAMP和ChABC的组合被确定为比单独使用任一种药物治疗SCI更有效。这些发现证明了使用PPC电纺微纤维用于SCI的多药物联合治疗的可行性。
This study describes the use of poly(propylene carbonate) (PPC) electrospun microfibres impregnated with a combination of dibutyryl cyclic adenosine monophosphate (db-cAMP) and chondroitinase ABC (ChABC) in the treatment of right-side hemisected spinal cord injury (SCI). Release of db-cAMP and/or ChABC from the microfibres was assessed in vitro using high-performance liquid chromatography (HPLC). Drug-impregnated microfibres were implanted into the hemisected thoracic spinal cord of rats, and treatment was evaluated using functional recovery examinations and immunohistochemistry. Our results demonstrated that the microfibres containing db-cAMP and/or ChABC displayed a stable and prolonged release of each agent. Sustained delivery of db-cAMP and/or ChABC was found to promote axonal regenerative sprouting, functional recovery, and reduced glial scar formation when compared to untreated control animals. The combination of both db-cAMP and ChABC was determined to be more effective than using either drug alone in the treatment of SCI. These findings demonstrate the feasibility of using PPC electrospun microfibres for multi-drug combination therapy in SCI.