Novel urine hepcidin assay by mass spectrometry
Novel urine hepcidin assay by mass spectrometry
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DOI：
10.1182/blood-2005-05-1873
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发表时间：
2005-11-01
期刊：
影响因子：
20.3
通讯作者：
Swinkels, D
中科院分区：
文献类型：
--
作者：
Kemna, E;Tjalsma, H;Swinkels, D
The hepatic peptide hormone hepcidin is the central regulator of iron metabolism and mediator of anemia of inflammation. To date, only one specific immuno-dot assay to measure hepcidin in urine had been documented. Here we report an alternative approach for quantification of hepcidin in urine by surf ace-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS). Peptide peaks were detected corresponding to the 3 forms of hepcidin normally found in urine. The identity of the peptide peak equivalent to hepcidin-25 was confirmed using synthetic human hepcidin-25. Validation of our MS data on samples with various hepcidin levels showed a strong correlation with previous immuno-clot assay results (Spearman R = 0.9275, P
