SOLUBILIZATION OF H‐2 HISTOCOMPATIBILITY ANTIGENS OF THE MOUSE BY TRITON X‐100 AND BUTANOL
SOLUBILIZATION OF H‐2 HISTOCOMPATIBILITY ANTIGENS OF THE MOUSE BY TRITON X‐100 AND BUTANOL
复制标题
TRITON X-100 和丁醇溶解小鼠 H-2 组织相容性抗原
作者：
T. Harris;S. Harris;C. A. Ogburn
用TritonX-100和正丁醇增溶BALB/c和CBA小鼠脾和肝细胞膜碎片中的组织相容性抗原（HCA）。这些可溶性制剂在细胞毒性和适当刺激同种脾细胞抑制抗体形成两项生物学试验中均能抑制或中和同种抗体（CBA抗BALB或BALB抗CBA）。脾衍生制剂的中和活性，每毫克，约10倍，来自肝脏，丁醇或木瓜蛋白酶提取的制剂比Triton X-100提取的高得多的比活性。所有制剂均含有不同比例的Sephadex G-200和其他非排除的活性分子种类。这两种Sephadex组分都可以吸附到绵羊红细胞（SRBC）上，但只有G-200排除材料的吸附才能使SRBC被CBA抗BALB抗体凝集;非排除组分可以与排除组分竞争，以防止这种抗体对包被SRBC的凝集。可溶性抗原在两种血清学试验中均能抑制同种抗体的产生，即所述的吸附血凝（AH）反应和CBA抗BALB抗体对BALB红细胞的标准血凝试验。
Histocompatibility antigens (HCA) have been solubilized by the use of Triton X-100 and n-butanol from cell membrane fragments of spleens and livers of BALB/c and CBA mice. These soluble preparations could inhibit or neutralize allogeneic antibody (CBA anti-BALB or BALB anti-CBA) in two biological tests, cytotoxicity and the suppression of antibody formation by appropriately stimulated spleen cells of the same strain. The neutralizing activities of the spleen-derived preparations, per milligram, were about 10 times those derived from liver, and preparations extracted by butanol or by papain were of substantially higher specific activity than those extracted by Triton X-100. All preparations contained active molecular species excluded by Sephadex G-200 and others, nonexcluded, in various proportions. Both Sephadex fractions could be adsorbed to sheep red blood cells (SRBC) but only the adsorption of the G-200-excluded material rendered the SRBC agglutinable by CBA anti-BALB antibodies; the non-excluded fraction could compete with the excluded fraction to prevent agglutination of the coated SRBC by such antibody. The soluble antigen could inhibit the allogeneic antibody in two serological tests, the adsorption hemagglutination (AH) reaction referred to, and the standard hemagglutination test of BALB RBC by CBA anti-BALB antibody.