Hoxb8 conditionally immortalised macrophage lines model inflammatory monocytic cells with important similarity to dendritic cells
Hoxb8 conditionally immortalised macrophage lines model inflammatory monocytic cells with important similarity to dendritic cells
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DOI：
10.1002/eji.201040962
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发表时间：
2011-02-01
影响因子：
5.4
通讯作者：
Taylor, Philip R.
中科院分区：
文献类型：
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作者：
Rosas, Marcela;Osorio, Fabiola;Taylor, Philip R.
我们研究了从条件永生化的小鼠骨髓前体细胞中产生真正巨噬细胞(MO)的可能性。Mo可以从Hoxb8条件永生化的巨噬细胞前体细胞系(MOP)中获得，使用M-CSF或GM-CSF。在GM-CSF(GM-MOP)中分化后的细胞在形态表型、抗原表型和对微生物刺激的功能反应等方面与GM-CSF骨髓来源的树突状细胞(BMDC)相似。尽管这两种细胞类型之间有很高的相似性，而且GM-MOP能够有效地将抗原递送给T细胞杂交瘤，但这些细胞在从初始的CD4(+)T细胞启动干扰素-伽马反应的扩张方面相对较差。转基因或基因异常小鼠的MOP的产生为研究GM-MOP的炎症作用提供了一个极好的机会，并减少了许多研究中对小鼠克隆的需求。因此，在GM-CSF中条件永生化的MOPS的分化代表了一种独特的炎性单核细胞样细胞的体外模型，与骨髓来源的树突状细胞有重要的区别，这将有助于与许多炎性单核细胞的亚表型相关的功能研究。
We have examined the potential to generate bona fide macrophages (MO) from conditionally immortalised murine bone marrow precursors. MO can be derived from Hoxb8 conditionally immortalised macrophage precursor cell lines (MOP) using either M-CSF or GM-CSF. When differentiated in GM-CSF (GM-MOP) the resultant cells resemble GM-CSF bone marrow-derived dendritic cells (BMDC) in morphological phenotype, antigen phenotype and functional responses to microbial stimuli. In spite of this high similarity between the two cell types and the ability of GM-MOP to effectively present antigen to a T-cell hybridoma, these cells are comparatively poor at priming the expansion of IFN-gamma responses from naive CD4(+) T cells. The generation of MOP from transgenic or genetically aberrant mice provides an excellent opportunity to study the inflammatory role of GM-MOP, and reduces the need for mouse colonies in many studies. Hence differentiation of conditionally immortalised MOPs in GM-CSF represents a unique in vitro model of inflammatory monocyte-like cells, with important differences from bone marrow-derived dendritic cells, which will facilitate functional studies relating to the many 'sub-pheno-types' of inflammatory monocytes.