Yorkie Negatively Regulates the Expression of Antimicrobial Proteins by Inducing Cactus Transcription in Prawns Macrobrachium nipponense.
Yorkie Negatively Regulates the Expression of Antimicrobial Proteins by Inducing Cactus Transcription in Prawns Macrobrachium nipponense.
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DOI：
10.3389/fimmu.2022.828271
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发表时间：
2022
影响因子：
7.3
通讯作者：
Ren Q
中科院分区：
文献类型：
--
作者：
Huang Y;Si Q;Du J;Ren Q
Hippo信号通路控制果蝇和哺乳动物的器官大小和免疫系统。Yorkie在Hippo通路中充当转录共激活剂，并与其他重要通路进行交叉对话。本研究从东方对虾（Macrobrachiumnipponense）中分离并鉴定了Yorkie基因和两种Cactus亚型（分别命名为MnYorkie、MnCactus-a和MnCactus-b）。结果表明，MnYorkie基因包含1620 bp的开放阅读框，编码539个氨基酸。MnCactus-a（377 aa）和MnCactus-b（471 aa）通过选择性剪接产生。MnYorkie和MnCactus在所有选择的组织中连续表达。在革兰氏阳性菌金黄色葡萄球菌和革兰氏阴性菌副溶血性弧菌的刺激下，血细胞和肠道中MnYorkie和MnCactus的mRNA水平经历时间依赖性增强。RNA干扰研究表明，MnYorkie沉默显着下调MnCactus的转录，但上调7个免疫相关基因的表达。此外，MnYorkie基因的体内沉默降低了对虾对细菌攻击的敏感性。继S.金黄色葡萄球菌和副溶血性弧菌感染后，对虾的存活率在2 ~ 6天显著提高，这与MnYorkie敲除期相对应。这些结果表明，Hippo通路中的MnYorkie可能在M.通过负调控免疫相关基因的表达，促进MnCactus的转录，从而抑制MnCactus的表达。
The Hippo signaling pathway controls organ size and immune system in Drosophila and mammals. Yorkie acts as a transcriptional co-activator in the Hippo pathway and cross-talks with other essential pathways. In this study, a Yorkie gene and two Cactus isoforms (designated as MnYorkie, MnCactus-a, and MnCactus-b, respectively) were isolated and characterized from oriental river prawns (Macrobrachium nipponense). Results showed that MnYorkie includes 1620 bp open reading frame and encodes a protein of 539 amino acids (aa). MnCactus-a (377 aa) and MnCactus-b (471 aa) were produced by alternative splicing. MnYorkie and MnCactus were continuously expressed in all selected tissues. Upon Gram-positive bacterium Staphylococcus aureus and Gram-negative bacterium Vibrio parahaemolyticus stimulation, the mRNA levels of MnYorkie and MnCactus in hemocytes and intestines underwent time-dependent enhancement. RNA interference studies showed that MnYorkie silencing remarkably downregulated the transcription of MnCactus but upregulated the expression of seven immune-related genes. In addition, MnYorkie silencing in vivo decreased the susceptibility of prawns to bacterial challenge. After S. aureus and V. parahaemolyticus infection, the survival rate of prawns increased significantly from 2 to 6 days, which corresponded to the period of MnYorkie knockdown. All these findings suggested that MnYorkie in the Hippo pathway might exhibit remarkable biological roles in the immune defense of M. nipponense by negatively regulating the expression of immune-related genes and promoting the transcription of MnCactus.