OsWRKY62 is a negative regulator of basal and Xa21-mediated defense against Xanthomonas oryzae pv. oryzae in rice
OsWRKY62 is a negative regulator of basal and Xa21-mediated defense against Xanthomonas oryzae pv. oryzae in rice
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DOI：
10.1093/mp/ssn024
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发表时间：
2008-05-01
期刊：
影响因子：
27.5
通讯作者：
Ronald, Pamela C.
中科院分区：
文献类型：
--
作者：
Peng, Ying;Bartley, Laura E.;Ronald, Pamela C.
水稻Xa21基因，它赋予对细菌病原体黄单胞菌致病型的抗性。Xoo是一种受体样激酶。参与Xa21介导的防御反应转导的几个组件尚未被确定。在这里，我们报告说，XA21结合WRKY转录因子，称为OsWRKY62。OsWRKY62基因编码两种剪接变体（OsWRKY62.1和OsWRKY62.2）。OsWRKY62.1：smGFP 2和OsWRKY62.2：smGFP 2融合蛋白部分定位于核。过量表达OsWRKY62.1的转基因植物在基础防御和Xa21介导的对Xoo的抗性方面受到损害。此外，OsWRKY62.1的过表达抑制了防御相关基因的激活。这些结果表明OsWRKY62是水稻先天免疫的负调节因子，是基础防御反应和小种特异性防御反应的重要介质。
The rice Xa21 gene, which confers resistance to the bacterial pathogen Xanthomonas oryzae pv. oryzae (Xoo), encodes a receptor-like kinase. Few components involved in transducing the Xa21-mediated defense response have yet been identified. Here, we report that XA21 binds to a WRKY transcription factor, called OsWRKY62. The OsWRKY62 gene encodes two splice variants (OsWRKY62.1 and OsWRKY62.2). OsWRKY62.1:smGFP2 and OsWRKY62.2: smGFP2 fusion proteins partially localize to the nucleus. Transgenic plants overexpressing OsWRKY62.1 are compromised in basal defense and Xa21-mediated resistance to Xoo. Furthermore, overexpression of OsWRKY62.1 suppresses the activation of defense-related genes. These results imply that OsWRKY62 functions as a negative regulator of innate immunity in rice, and serves as a critical mediator of both basal and race-specific defense responses.