Absorption and fluorescence spectroscopic investigation of indocyanine green
Absorption and fluorescence spectroscopic investigation of indocyanine green
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DOI：
10.1016/1010-6030(95)04292-x
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发表时间：
1996-05-31
影响因子：
4.3
通讯作者：
Abels, C
中科院分区：
文献类型：
--
作者：
Philip, R;Penzkofer, A;Abels, C
测定了吲哚菁绿在甲醇、水和白蛋白水溶液中的吸收光谱、荧光量子分布、荧光量子产率和荧光极化度随染料浓度的变化。单体荧光量子产率被限制在几个百分点的内部转换。在水中，二聚化始于低浓度(约3 × 10(-8) mol dm(-3))，并降低荧光量子产率。染料对白蛋白的强亲和力将二聚化的发生转移到更高的浓度。在甲醇中，二聚化作用较弱，在高浓度时形成紧密间隔的对。实验和理论分析了染料对白蛋白的吸附和染料在溶剂中的聚集。
Absorption spectra, fluorescence quantum distributions, fluorescence quantum yields and degrees of fluorescence polarization vs, dye concentration were determined for indocyanine green in methanol, water and aqueous albumin solution. The monomer fluorescence quantum yield is limited to a few per cent by internal conversion. In water, dimerization starts at low concentrations (approximately 3 x 10(-8) mol dm(-3)) and lowers the fluorescence quantum yield. The strong affinity of the dye to albumin shifts the onset of dimerization to higher concentrations. in methanol, dimerization is weak and closely spaced pair formation dominates at high concentrations. Dye adsorption to albumin and dye aggregation in the solvents were analysed experimentally and theoretically.