Drag-reducing polymers diminish near-wall concentration of platelets in microchannel blood flow.
Drag-reducing polymers diminish near-wall concentration of platelets in microchannel blood flow.
复制标题
DOI：
10.3233/bir-2010-0570
复制
发表时间：
2010
期刊：
影响因子：
1.1
通讯作者：
Kameneva MV
中科院分区：
文献类型：
--
作者：
Zhao R;Marhefka JN;Antaki JF;Kameneva MV
血小板在血管壁或人工表面附近的聚集是导致凝血和/或血栓形成的一系列事件中的一个重要因素。在小血管和流动通道中，这种现象被归因于血液相分离，在壁上创造了一层缺乏红细胞(RBC)的层。我们推测，血液可溶性减阻聚合物(DRP)先前被证明可以减少小通道中的近壁红细胞耗尽层，因此可能减少近壁血小板的过剩。本研究调查了DRP对血小板大小的荧光颗粒(DIAM)横向分布的影响。=2微米，2.5×108/毫升)在玻璃正方形微通道中(宽度和深度=100微米)。红细胞悬浮液在PBS中与颗粒混合，以6-18ml/h的流速通过微通道，在有或没有添加DRP的情况下(加入10ppm的PEO，相对分子质量=4500 kDa)。微观流动显示，在所有测试流量下，对照样品的近壁区颗粒浓度升高(6ml/h时为2.4±0.8倍，18ml/h时为3.3±0.3倍)。添加微量浓度的DRP几乎消除了近壁颗粒的过剩，有效地导致它们在整个通道中均匀分布，表明DRP在管理和减轻血栓形成方面具有潜在的重要作用。
The accumulation of platelets near the blood vessel wall or artificial surface is an important factor in the cascade of events responsible for coagulation and/or thrombosis. In small blood vessels and flow channels this phenomenon has been attributed to the blood phase separation that creates a red blood cell (RBC)-poor layer near the wall. We hypothesized that blood soluble drag-reducing polymers (DRP), which were previously shown to lessen the near-wall RBC depletion layer in small channels, may consequently reduce the near-wall platelet excess. This study investigated the effects of DRP on the lateral distribution of platelet-sized fluorescent particles (diam. = 2 µm, 2.5 × 108/ml) in a glass square microchannel (width and depth = 100 µm). RBC suspensions in PBS were mixed with particles and driven through the microchannel at flow rates of 6–18 ml/h with and without added DRP (10 ppm of PEO, MW = 4500 kDa). Microscopic flow visualization revealed an elevated concentration of particles in the near-wall region for the control samples at all tested flow rates (between 2.4 ± 0.8 times at 6 ml/h and 3.3 ± 0.3 times at 18 ml/h). The addition of a minute concentration of DRP virtually eliminated the near-wall particle excess, effectively resulting in their even distribution across the channel, suggesting a potentially significant role of DRP in managing and mitigating thrombosis.