The Fd-GOGAT1 mutant gene lc7 confers resistance to Xanthomonas oryzae pv. Oryzae in rice.
The Fd-GOGAT1 mutant gene lc7 confers resistance to Xanthomonas oryzae pv. Oryzae in rice.
复制标题
Fd-GOGAT1 突变基因 lc7 赋予对米黄单胞菌 pv 的抗性。
DOI：
10.1038/srep26411
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发表时间：
2016-05-23
影响因子：
4.6
通讯作者：
Zhai W
中科院分区：
文献类型：
--
作者：
Chen H;Li C;Liu L;Zhao J;Cheng X;Jiang G;Zhai W
抗病是作物改良的重要目标。抗性的分子机制有待进一步研究。在这里，我们报告了一个水稻叶色突变体Lc7的鉴定，该突变体在叶绿素合成和光合作用方面存在缺陷，但对水稻白叶枯病菌具有抗性。大米(Xoo)。基于图谱的克隆表明，LC7编码一个突变的铁氧还蛋白依赖谷氨酸合成酶1(Fd-GOGAT1)。Fd-GOGAT1在提高氮素利用效率方面具有很大潜力，但其在细菌耐药性中的作用尚未见报道。Lc7突变体在叶片中积累了过量的ROS(活性氧物种)，导致叶片颜色在四叶期后变黄。与野生型相比，LC7突变体对7个Xoo菌株具有广谱高抗性。差异表达基因(Degs)和qRT-PCR分析表明，在Lc7突变体中，参与这种广谱抗性的许多防御途径被激活。这些结果表明，Fd-GOGAT1除了调节氮素同化和叶绿体发育外，还在广谱白叶枯病抗性中发挥重要作用。
Disease resistance is an important goal of crop improvement. The molecular mechanism of resistance requires further study. Here, we report the identification of a rice leaf color mutant, lc7, which is defective in chlorophyll synthesis and photosynthesis but confers resistance to Xanthomonas oryzae pv. Oryzae (Xoo). Map-based cloning revealed that lc7 encodes a mutant ferredoxin-dependent glutamate synthase1 (Fd-GOGAT1). Fd-GOGAT1 has been proposed to have great potential for improving nitrogen-use efficiency, but its function in bacterial resistance has not been reported. The lc7 mutant accumulates excessive levels of ROS (reactive oxygen species) in the leaves, causing the leaf color to become yellow after the four-leaf stage. Compared to the wild type, lc7 mutants have a broad-spectrum high resistance to seven Xoo strains. Differentially expressed genes (DEGs) and qRT-PCR analysis indicate that many defense pathways that are involved in this broad-spectrum resistance are activated in the lc7 mutant. These results suggest that Fd-GOGAT1 plays an important role in broad-spectrum bacterial blight resistance, in addition to modulating nitrogen assimilation and chloroplast development.