Identification and isolation of a spiroplasma pathogen from diseased freshwater prawns, Macrobrachium rosenbergii, in China: A new freshwater crustacean host
Identification and isolation of a spiroplasma pathogen from diseased freshwater prawns, Macrobrachium rosenbergii, in China: A new freshwater crustacean host
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DOI：
10.1016/j.aquaculture.2011.03.018
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发表时间：
2011-07
期刊：
影响因子：
4.5
通讯作者：
Tingming Liang;Xin-lun Li;Jie Du;Wei Yao;G. Sun;Xuehong Dong;Zhiguo Liu;Jiangtao Ou;Q. Meng;W. Gu;Wen Wang
中科院分区：
文献类型：
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作者：
Tingming Liang;Xin-lun Li;Jie Du;Wei Yao;G. Sun;Xuehong Dong;Zhiguo Liu;Jiangtao Ou;Q. Meng;W. Gu;Wen Wang
由于淡水养殖业的集约化发展及其对大量死亡的敏感性，包括淡水虾（罗氏沼虾）在内的淡水养殖甲壳类动物的疾病流行最近在中国江苏受到高度关注。形态学观察表明，病原菌存在于患病M．罗氏沼虾具有螺旋形态并且缺乏细胞壁。该制剂可感染血细胞以及肝胰腺、消化道和心肌等器官的所有结缔组织。该试剂能够通过孔径为 220 nm 的膜过滤器，并且可以在 R2 培养基中培养。采用科赫定律评价该病原体的致病性，通过实验感染确定该病原体产生的死亡率为80%。通过分析 16S rRNA 完整序列来研究系统发育位置。结果表明，该病原菌与其他淡水螺原体亲缘关系较近，与Mirum螺原体同属一个系统发育树。总而言之，研究结果表明，从患病M中分离出病原体。罗氏沼虾是一种螺原体微生物。这是商业开发的甲壳类动物中螺原体病原体的第四份报告，表明螺原体病原体已在水生甲壳类动物中迅速传播；因此，需要更多的研究关注。
Disease epizootics in freshwater cultured crustaceans, including freshwater prawns (Macrobrachium rosenbergii), gained high attention recently in Jiangsu, China due to intensive development of freshwater aquaculture and their susceptibility to massive mortalities. Morphological observation indicated that the pathogen in diseasedM. rosenbergiihad a helical morphology and lacked a cell wall. The agent could infect hemocytes and all the connective tissues of the organs such as hepatopancreas, digestive tract, and cardiac muscle. The agent was able to pass through membrane filters with pores 220 nm in diameter and could be cultivated by R2 medium. The pathogenicity of the agent was evaluated by Koch's postulate and the mortalities produced by the pathogen were determined to be 80% through experimental infection. The phylogenetic position was investigated by analysis of the 16S rRNA complete sequence. The result revealed that the pathogen had a close relationship with other freshwater spiroplasmas and was situated in the Mirum clade together withSpiroplasma mirumin a phylogenetic tree. Taken in total, the findings demonstrated that the pathogen isolated from diseasedM. rosenbergiiwas a spiroplasma microorganism. This is the fourth report of a spiroplasma pathogen in commercially exploited crustaceans and indicates that spiroplasma pathogens have spread rapidly in aquatic crustaceans; thus, requiring more substantial research attention.