Requirement of protein synthesis for the degradation of host mRNA in Friend erythroleukemia cells infected wtih herpes simplex virus type 1
Requirement of protein synthesis for the degradation of host mRNA in Friend erythroleukemia cells infected wtih herpes simplex virus type 1
复制标题
感染 1 型单纯疱疹病毒的 Friend 红白血病细胞中宿主 mRNA 降解所需的蛋白质合成要求
作者：
Y. Nishioka;S. Silverstein
我们描述的实验表明，感染后不久的朋友红白血病细胞与单纯疱疹病毒（HSV），多聚核糖体解离和细胞的mRNA降解。蔗糖密度梯度上的感染细胞提取物的分析表明，大多数的多核糖体已解离为monoribosomes在2小时postinfection。感染细胞RNA的物理测量支持这一结论，并表明聚腺苷酸化RNA的大小减少。mRNA的降解显然是一个随机过程，这是通过当在不同时间从感染细胞中提取的多聚腺苷酸化RNA与珠蛋白互补DNA杂交时未能检测到Crt 1/2的位移来判断的。在旨在确定多聚核糖体的解离是否足以引起珠蛋白mRNA降解的实验中，当用浓度足以解离所有多聚核糖体的NaF或帕塔霉素处理细胞时，未感染细胞中珠蛋白mRNA的量没有变化。在细胞感染紫外线照射的病毒多核糖体解离，但珠蛋白mRNA不降解，这表明它是可能的分离解离降解。
We describe experiments which demonstrate that shortly after infection of Friend erythroleukemia cells with herpes simplex virus (HSV), polyribosomes dissociate and cellular mRNA degrades. Analysis of infected cell extracts on sucrose density gradients demonstrates that the majority of the polyribosomes have dissociated to monoribosomes at 2 h postinfection. Physical measurements of infected-cell RNAs support this conclusion and demonstrate that the polyadenylated RNAs decrease in size. The degradation of mRNA is apparently a stochastic process as judged by the failure to detect a shift in the Crt1/2 when polyadenylated RNA extracted from infected cells at different times is hybridized to globin complementary DNA. In experiments designed to determine whether dissociation of polyribosomes is sufficient to cause degradation of globin mRNA, the amount of globin mRNA in uninfected cells did not change when cells were treated with NaF or pactamycin at concentrations sufficient to dissociate all polyribosomes. In cells infected with UV-irradiated virus polyribosomes dissociate but globin mRNA does not degrade, suggesting that it is possible to separate dissociation from degradation.