A two-dimensional immunomagnetic nano-net for the efficient isolation of circulating tumor cells in whole blood
A two-dimensional immunomagnetic nano-net for the efficient isolation of circulating tumor cells in whole blood
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DOI：
10.1039/c9nr06256d
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发表时间：
2019-11-28
期刊：
影响因子：
6.7
通讯作者：
Chang, Ying-Chih
中科院分区：
文献类型：
--
作者：
Lai, Chian-Hui;Tsai, Wen-Sy;Chang, Ying-Chih
设计并合成了免疫磁性“纳米网”，用于从混合物中特异性捕获感兴趣的稀有细胞。纳米网 Ab@Lipo-MNP-GO 由脂质涂覆的磁性纳米颗粒-氧化石墨烯片复合物上的缀合抗体分子组成。通过 SQUID、FTIR、TGA、DLS 和 SEM 对构建体及其前体的磁性、化学成分和形态进行了表征，以确认合成步骤的可行性以及适用于细胞捕获的液相免疫识别的所得特性。当应用于捕获口腔癌、结肠癌和肺癌临床患者血液样本中的循环肿瘤细胞（CTC）时，纳米网结构表现出远远优越的能力，而传统的免疫磁珠在某些情况下无法捕获任何CTC，即使增加磁珠浓度也是如此。共焦图像显示，纳米网包裹在 CTC 周围，而免疫磁珠通过点接触将其附着。稳定的贴片状多价基质纳米网被证明可以解决免疫磁珠与靶细胞单点接触的缺点。该策略对于复杂流体中的任何细胞分离都是通用的。
An immunomagnetic "nano-net" was designed and synthesized for specifically capturing rare cells of interest from mixtures. The nano-net, Ab@Lipo-MNP-GO, consists of conjugated antibody molecules on a lipid coated magnetic nanoparticle-graphene oxide sheet complex. The magnetism, chemical composition, and the morphology of the construct and its precursors were characterized by SQUID, FTIR, TGA, DLS and SEM, to confirm the feasibility of the synthetic steps and the resulting properties suitable for solution phase immuno-recognition for cell capture. When applied to capturing circulating tumor cells (CTCs) in oral, colon and lung cancer clinical patients' blood samples, the nano-net construct exhibited far superior ability whereas conventional immunomagnetic beads in some cases were unable to capture any CTCs, even by increasing the bead concentration. Confocal images showed that the nano-net wrapped around the CTCs while the immunomagnetic beads attached them with point contacts. A stable, patch-like multivalent matrix nano-net was demonstrated to tackle the shortcomings of single point contact of immunomagnetic beads to the target cell. This strategy is universal for any cell separation in complex fluids.