Anisakis simplex allergy after eating chicken meat.
Anisakis simplex allergy after eating chicken meat.
复制标题
吃鸡肉后单纯异尖线虫过敏。
DOI：
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复制
发表时间：
2006
影响因子：
7.2
通讯作者：
C. Martínez
中科院分区：
文献类型：
--
作者：
A. Armentia;F. Martín;C. Pascual;M. Martín‐Esteban;A. Callejo;C. Martínez
背景
对食物的过敏反应可由诱发致敏的污染物产生。其中，单纯异尖线虫可引起海鲜感染，过敏症状（荨麻疹-血管性水肿，过敏反应和哮喘）可在食用或处理受影响的鱼类后出现。虽然海产品是人类感染这种寄生虫的主要来源，但我们发现8名先前诊断为A型单纯性致敏的患者在食用鸡肉后出现过敏症状。鸡饲料通常含有高比例的鱼粉，可能受到这种线虫的污染。
目的
我们研究的目的是确定鸡肉中存在的寄生虫蛋白是否可能导致这些受试者报告的症状。
方法
我们对这8例患者进行了体内试验（针刺、支气管激发和双盲安慰剂对照肉鸡激发）。我们使用来自8名患者和对照的血清进行免疫印迹，以检测单纯形抗原致敏。我们还调查了A单纯蛋白的存在下，从鸡的血清中喂养鱼粉和其他血清中只喂养谷物的鸡。我们通过血清学方法排除了对其他鸡线虫的致敏性。
结果
所有8例患者均表现为单抗点刺和激发试验阳性。当我们用鱼粉喂养的鸡的血清作为抗原进行印迹时，患者3、4、5、6、7和8识别出16 kd的条带，这也是用鱼贝类和A单形幼虫池时获得的。未观察到仅用谷物喂养的鸡的血清。
结论
我们提供的证据，在体内和体外试验的基础上，受试者高度致敏A单纯可以检测到异尖线虫属过敏原的鸡肉中的存在。
BACKGROUND
Allergic reactions to food can be produced by contaminants that induce sensitization. Among these, Anisakis simplex can cause seafood infestation, and allergic symptoms (urticaria-angioedema, anaphylaxis, and asthma) can follow the eating or handling of affected fish. Although seafood is the principal source of human infections by this parasite, we have found allergic symptoms in 8 patients previously diagnosed as having A simplex sensitization after they ate chicken meat. Chicken feed usually has a high proportion of fishmeal, which might possibly be contaminated by this nematode.
OBJECTIVE
The aim of our study was to determine whether parasite proteins present in chicken meat could be responsible for the symptoms reported by these subjects.
METHODS
We carried out in vivo tests (prick, bronchial challenge, and double-blind placebo-controlled challenge with meat chicken) in these 8 patients. We performed immunoblotting using the sera from the 8 patients and controls in order to detect A simplex sensitization. We also investigated the presence of A simplex proteins in sera from chickens fed with fishmeal and in other sera from chickens fed only with cereals. We excluded sensitization to other chicken nematodes by serologic methods.
RESULTS
All 8 patients presented positive prick and challenges to A simplex. When we used serum from chickens fed with fishmeal as the antigen in blotting, patients 3, 4, 5, 6, 7, and 8 recognized a band of 16 kd, also obtained when using pools of fish-shellfish and A simplex larva. No detection was observed with sera from chickens fed with only cereals.
CONCLUSION
We provide evidence, based on in vivo and in vitro tests, that subjects highly sensitized to A simplex can detect the presence of Anisakis species allergens in chicken meat.
单纯异尖线虫主要过敏原的纯化和分子克隆
DOI：
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发表时间：
2004
期刊：
Mol.Biochem.Parasitol. 135(1)
影响因子：
--
作者：
加藤亮;志村もと子;黒田久雄;中曽根英雄;Kuniyoshi Shimakura
通讯作者：
Kuniyoshi Shimakura