Association of fatty acids and lipids metabolism in placenta with early spontaneous pregnancy loss in Chinese women
Association of fatty acids and lipids metabolism in placenta with early spontaneous pregnancy loss in Chinese women
复制标题
胎盘脂肪酸和脂质代谢与中国女性早期自然妊娠流产的关系
DOI：
10.1039/c7fo01545c
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发表时间：
2018-02-01
期刊：
影响因子：
6.1
通讯作者：
Li, Duo
中科院分区：
文献类型：
--
作者：
Li, Kelei;Zhang, Xiaotian;Li, Duo
本研究旨在探讨胎盘脂肪酸和脂质代谢与中国妇女早期自然流产（ESPL）的关系。将70例ESPL患者和29例要求法律的人工流产的健康孕妇分别纳入病例组和对照组。病例组和对照组中受试胎仔的胎龄范围为4 - 10周。采用气相色谱法测定蜕膜和绒毛组织中的总脂肪酸组成。采用超高效液相色谱-四极杆飞行时间质谱（UPLC-QTOFMS）对蜕膜和绒毛组织进行代谢组学分析。病例组蜕膜和绒毛组织中总C18：3 n-3、蜕膜组织中总n-3多不饱和脂肪酸（n-3 PUFA）和绒毛组织中总C18：2n-6均显著低于对照组。病例组绒毛组织C20：4 n-6/C20：5 n-3比值显著高于对照组，而前列腺素I-2、羟二十碳五烯酸、白三烯B-5和血栓素B-3显著低于对照组。此外，蜕膜和绒毛组织中溶血磷脂含量低，绒毛组织中甘油二酯含量低也与ESPL的发生有关。结论：胎盘中必需脂肪酸缺乏、C20：4 n-6/C20：5 n-3比值高、类花生酸代谢异常、溶血磷脂和甘油二酯含量低均为ESPL的潜在危险因素。
The aim of the present study was to evaluate the association of fatty acids and lipids metabolism in placenta with early spontaneous pregnancy loss (ESPL) in Chinese women. Seventy women with ESPL and 29 healthy pregnant women who asked for legal induced abortion were included in the case and control groups, respectively. The gestational age of the subject foetuses in both the case and control groups ranged from 4 to 10 weeks. The total fatty acids composition in the decidual and villous tissues was detected by gas-liquid chromatography using a standard method. Metabonomics analysis of the decidual and villous tissues was conducted by ultra-performance liquid chromatography quadrupole time of flight mass spectrometry (UPLC-QTOFMS). The total C18:3n-3 in the decidual and villous tissues, total n-3 polyunsaturated fatty acid (n-3 PUFA) in the decidual tissue, and total C18:2n-6 in the villous tissue were all significantly lower in the case group than in the control group. The ratio of C20:4n-6/C20:5n-3 in villous tissue was significantly higher, but prostaglandin I-2 as well as hydroxyeicosapentaenoic acid, leukotriene B-5 and thromboxane B-3 in the villous tissue were significantly lower in the case group than in the control group. In addition, the low content of lysophosphatide in the decidual and villous tissues and the low content of diacylglycerol in the villous tissue were also associated with the occurance of ESPL. In conclusion, the lack of essential fatty acids, high ratio of C20:4n-6/C20:5n-3, abnormal eicosanoids metabolism and low content of lysophosphatide and diacylglycerol in the placenta were all potential risk factors for ESPL in Chinese.