Future application of hair follicle stem cells: capable in differentiation into sweat gland cells
Future application of hair follicle stem cells: capable in differentiation into sweat gland cells
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DOI：
10.3760/cma.j.issn.0366-6999.20130931
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发表时间：
2013-09-20
影响因子：
6.1
通讯作者：
Fu Xiao-bing
中科院分区：
文献类型：
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作者：
Wang Yao;Liu Zhi-yue;Fu Xiao-bing
背景在严重的皮肤损伤中，汗腺（SGs）在完全破坏后无法再生，因此寻找理想的干细胞来源以再生功能性的SGs是非常重要的。毛囊干细胞具有成体干细胞的明显特性，具有多能性和易获取性。在本研究中，我们试图通过体外合作共培养系统，引导汗腺细胞（SGCs）特异性分化的HFSCs。方法设计的transwell共培养微环境由两个关键因素组成：热休克SGCs和真皮样间充质组织，这两个因素在两个对照组中独立出现；诱导后，将纯化的诱导sgc样细胞移植至裸鼠全层烫伤创面，4周后通过免疫组织化学和免疫荧光分析鉴定重建的sgc样细胞结构。结果实验组部分HFSCs最终表达了SGCs表型，而仅含有真皮样间充质组织的对照组1失败，热休克SGCs组成的对照组2效率较差；免疫荧光染色和流式细胞术分析显示，HFSCs特异性生物标志物的表达下调，而SGCs特异性抗原的阳性效率反而升高；此外，诱导的SGCs高表达外胞浆异常素a （EDA）和外胞浆异常素a受体（EDAR）基因和蛋白；细胞移植后，最年轻的SGCs样结构形成，SGCs特殊抗原呈阳性，这在未治疗的伤口中从未发生过(P
Background Sweat glands (SGs) can not regenerate after complete destruction in the severe skin injury, so it is important to find a ideal stem cell source in order to regenerate functional SGs. Hair follicle stem cells (HFSCs) possess the obvious properties of the adult stem cells, which are multipotent and easily accessible. In this research, we attempted to direct the HFSCs suffered from the sweat gland cells (SGCs) special differentiation by a cooperative co-culture system in vitro.Methods The designed co-culture microenvironment in the transwell was consist of two critial factors: heat shocked SGCs and dermis-like mesenchymal tissue, which appeared independently in the two control groups; after induction, the purified induced SGC-like cells were transplanted into the full-thickness scalded wounds of the nude mice, after 4 weeks, the reconstructed SG-like structures were identified by immunohistochemical and immunofluorescence analysis.Results A part of HFSCs in experimental group finally expressed SGCs phenotypes, by contrast, the control group 1 which just containing dermis-like mesenchymal tissue failed and the control group 2 consisted of heat shocked SGCs was in a poor efficiency; by immunofluorescence staining and flow cytometry analysis, the expression of HFSCs special biomarkers was down regulated, instead of the positive efficiency of SGCs special antigens increased; besides, the induced SGCs displayed a high expression of ectodysplasin A (EDA) and ectodysplasin A receptor (EDAR) genes and proteins; after cell transplantation, the youngest SG-like structures formed and be positive in SGCs special antigens, which never happened in untreated wounds (P