Binding of heparin and heparan sulphate to rat liver cells.
Binding of heparin and heparan sulphate to rat liver cells.
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DOI：
10.1016/0006-291x(77)91384-5
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发表时间：
1977-01
影响因子：
3.1
通讯作者：
L. Kjellén;L. Kjellén;Åke Oldberg;Åke Oldberg;K. Rubin;K. Rubin;M. Höök;M. Höök
中科院分区：
文献类型：
--
作者：
L. Kjellén;L. Kjellén;Åke Oldberg;Åke Oldberg;K. Rubin;K. Rubin;M. Höök;M. Höök
证明了猪粘膜肝素和大鼠肝硫酸乙酰肝素与大鼠肝细胞的结合。该过程是依赖于时间，可逆和饱和。结合到细胞的肝素的最大量超过硫酸乙酰肝素的量，以摩尔计。这两种多糖的结合是特异性的，因为除了肝素相关的糖胺聚糖之外，过量的糖胺聚糖不影响结合反应。当在低温下进行孵育或在胰蛋白酶处理细胞后进行孵育时，肝素与细胞的结合显著减少。
The binding of pig mucosal heparin and rat liver heparan sulphate to rat liver cells is demonstrated. The process is shown to be time dependent, reversible and saturable. The maximal amount of heparin bound to the cells exceeds that of heparan sulphate, on a molar basis.The binding of both polysaccharides is specific, in that excess amounts of glycosaminoglycans other than heparin-related do not affect the binding reaction.The binding of heparin to cells was markedly reduced when incubations were performed at low temperature or after trypsin treatment of the cells.