Diet-induced obesity alters anabolic signalling in mice at the onset of skeletal muscle regeneration
Diet-induced obesity alters anabolic signalling in mice at the onset of skeletal muscle regeneration
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DOI：
10.1111/apha.12537
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发表时间：
2015-09-01
影响因子：
6.3
通讯作者：
Washington, T. A.
中科院分区：
文献类型：
--
作者：
Brown, L. A.;Lee, D. E.;Washington, T. A.
肥胖被归类为一种代谢紊乱，与损伤后肌肉再生延迟有关。为了最佳的骨骼肌再生，炎症、细胞外基质重塑和肌肉生长必须受到严格的调控。此外，再生过程依赖于成肌细胞增殖和分化的肌源性调节因子(MRF)的激活。本研究旨在探讨肥胖对小鼠肌肉再生时炎症和蛋白质合成信号及MRF表达的影响。方法48只雄性C57BL/6J小鼠(3周龄)随机分为高脂饮食(HFD，60%脂肪)和瘦饮食(10%脂肪)两组，每组12周。15周后，损伤组(n=5~8个/组)胫骨前肌(TA)注射布比卡因，对照组(n=5~6)注射PBS。注射后3天或28天切除TA。结果肥胖小鼠肌肉再生受损。肥胖小鼠IL-6、MyoD和IGF-1mRNA丰度较瘦小鼠显著降低(P
AimObesity is classified as a metabolic disorder that is associated with delayed muscle regeneration following damage. For optimal skeletal muscle regeneration, inflammation along with extracellular matrix remodelling and muscle growth must be tightly regulated. Moreover, the regenerative process is dependent on the activation of myogenic regulatory factors (MRFs) for myoblast proliferation and differentiation. The purpose of this study was to determine how obesity alters inflammatory and protein synthetic signalling and MRF expression at the onset of muscle regeneration in mice.MethodsForty-eight male C57BL/6J mice (3weeks old) were randomly assigned to either a high-fat diet (HFD, 60% fat) or a lean diet (10% fat) for 12weeks. At 15weeks, bupivacaine was injected into the tibialis anterior (TA) of the injured group (n=5-8/group) and PBS was injected into the control (n=5-6). The TA was excised 3 or 28days after injection.ResultsWe demonstrated impaired muscle regeneration in obese mice. The obese mice had reduced IL-6, MyoD and IGF-1 mRNA abundance compared to the lean mice (P