Bacillus subtilis biofilm matrix components target seed oil bodies to promote growth and anti-fungal resistance in melon.
Bacillus subtilis biofilm matrix components target seed oil bodies to promote growth and anti-fungal resistance in melon.
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枯草芽孢杆菌生物膜基质组分靶向种子油体以促进甜瓜的生长和抗真菌抗性。
DOI：
10.1038/s41564-022-01134-8
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发表时间：
2022-07
影响因子：
28.3
通讯作者：
中科院分区：
文献类型：
--
作者：
有益的微生物被用来刺激种子的萌发；然而，其促进生长的机制在很大程度上仍未被探索。枯草芽孢杆菌通常存在于不同的植物器官中，提供对病原体的保护或刺激植物生长。我们报道了在甜瓜种子中应用枯草杆菌引起的遗传和生理反应，改变了5天和1个月大的甜瓜种子萌发时的代谢和发育状态。我们分析了枯草杆菌生物膜细胞外基质中不同成分的突变株与种子的相互作用，发现在种子储存组织的细菌定植和促进生长方面存在合作。结合共聚焦显微镜和荧光探针，我们发现细胞外基质的两个特定成分，淀粉样蛋白Tasa和风霉素，不同地增加了种子内的活性氧浓度。此外，利用电子显微镜、荧光显微镜和代谢组学方法，我们发现Tasa和丰霉素都以种子胚乳中的油体为靶标，导致脂质代谢和谷胱甘肽相关分子积累的特异性变化。反过来，这又导致了两种不同的植物生长发育计划：TASA和风霉素刺激胚根的发育，而风霉素单独刺激成年植物的生长和叶面对灰霉病菌的抗性。了解细菌生长促进的机制将有助于设计定制的生长促进菌株。采用多重组学和显微分析相结合的方法，对枯草芽孢杆菌促进瓜子生长的机理进行了研究。
Beneficial microorganisms are used to stimulate the germination of seeds; however, their growth-promoting mechanisms remain largely unexplored. Bacillus subtilis is commonly found in association with different plant organs, providing protection against pathogens or stimulating plant growth. We report that application of B. subtilis to melon seeds results in genetic and physiological responses in seeds that alter the metabolic and developmental status in 5-d and 1-month-old plants upon germination. We analysed mutants in different components of the extracellular matrix of B. subtilis biofilms in interaction with seeds and found cooperation in bacterial colonization of seed storage tissues and growth promotion. Combining confocal microscopy with fluorogenic probes, we found that two specific components of the extracellular matrix, amyloid protein TasA and fengycin, differentially increased the concentrations of reactive oxygen species inside seeds. Further, using electron and fluorescence microscopy and metabolomics, we showed that both TasA and fengycin targeted the oil bodies in the seed endosperm, resulting in specific changes in lipid metabolism and accumulation of glutathione-related molecules. In turn, this results in two different plant growth developmental programmes: TasA and fengycin stimulate the development of radicles, and fengycin alone stimulate the growth of adult plants and resistance in the phylloplane to the fungus Botrytis cinerea. Understanding mechanisms of bacterial growth promotion will enable the design of bespoke growth promotion strains. Mechanisms of growth promotion of Bacillus subtilis on melon seeds are identified using a combination of multi-omics and microscopy.
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影响因子：
3
作者：
Falgueras J;Lara AJ;Fernández-Pozo N;Cantón FR;Pérez-Trabado G;Claros MG
通讯作者：
Claros MG
影响因子：
8.8
作者：
De Chirico, Simone;di Bari, Vincenzo;Gray, David
通讯作者：
Gray, David
影响因子：
5.5
作者：
Farzand, Ayaz;Moosa, Anam;Gao, Xuewen
通讯作者：
Gao, Xuewen
影响因子：
4.3
作者：
da Silva RR;Wang M;Nothias LF;van der Hooft JJJ;Caraballo-Rodríguez AM;Fox E;Balunas MJ;Klassen JL;Lopes NP;Dorrestein PC
通讯作者：
Dorrestein PC
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y