A correlation between the virulence and the adhesion of Listeria monocytogenes to silicon nitride: an atomic force microscopy study.
A correlation between the virulence and the adhesion of Listeria monocytogenes to silicon nitride: an atomic force microscopy study.
复制标题
单核细胞增生李斯特菌的毒力与氮化硅粘附之间的相关性：原子力显微镜研究。
DOI：
10.1016/j.colsurfb.2009.05.027
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
N. Abu
中科院分区：
文献类型：
--
作者：
Bong;Travis Haines;N. Abu
单核增生李斯特菌是一种兼性胞内革兰氏阳性细菌，广泛分布于环境中。尽管在物种水平上具有致病性，但单核增生乳杆菌实际上包括多种菌株，从可导致疾病和/或死亡的致病性菌株到其他相对无毒的菌株。目前研究的主要目的是回答一个问题，即单核增生乳杆菌与惰性表面的增强结合或附着是否与食源性分离株的致病性有任何关系。为了回答这个问题，使用原子力显微镜定量了8株不同致病性的单核增生乳杆菌菌株对氮化硅模型表面的纳米级粘附力。采用高致病性菌株（EGDe、874、1002、ATCC 19115）、中致病性菌株（ATCC 19112、ATCC 19118）和非致病性菌株（ATCC 15313、HCC25）。结果表明，菌株在小鼠体内的平均纳米级黏附力（nN）与50%致死剂量（LD50）呈对数相关关系(nN)=−0.032ln(LD50)+1.040, r2=0.96。这种相关性表明，纳米级黏附可能被用作区分强毒性和无毒单核细胞增生乳杆菌菌株的设计标准。最后，由氮化硅模拟的毒性菌株对惰性表面的更强粘附可能是致病菌株在环境中更好生存的一种方式，从而增加了它们感染动物或人类的可能性。
Listeria monocytogenes is a facultative intracellular Gram-positive bacterium that is widely distributed in the environment. Despite being pathogenic at the species level, L. monocytogenes in fact comprises a diversity of strains from pathogenic ones that can result in disease and/or mortality to others that are relatively avirulent. The main goal of the current study was to answer the question on whether enhanced binding or attachment of L. monocytogenes to inert surfaces bears any relationship to pathogenicity in food-borne isolates. To answer this question, the nanoscale adhesion forces of eight L. monocytogenes strains that vary in their pathogenicity levels to a model surface of silicon nitride were quantified using atomic force microscopy. The strains used were the highly pathogenic (EGDe, 874, 1002, ATCC 19115), the intermediate pathogenic (ATCC 19112, ATCC 19118), and the non pathogenic (ATCC 15313 and HCC25). Our results indicate that the average nanoscale adhesion (in nN) and the 50% lethal dose (LD50) of strain virulence quantified in mice are logarithmically correlated according to: (nN)=−0.032ln(LD50)+1.040, r2=0.96. Such correlation indicates that nanoscale adhesion could potentially be used as a design criterion to distinguish between virulent and avirulent L. monocytogenes strains. Finally, stronger adhesion of virulent strains to inert surfaces modeled by silicon nitride might be a way for pathogenic strains to survive better in the environment and thus increase their likelihood of infecting animals or humans.