Identification of risk for ovarian disease enhanced by BPB or BPAF exposure
Identification of risk for ovarian disease enhanced by BPB or BPAF exposure
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识别 BPB 或 BPAF 暴露增强的卵巢疾病风险
DOI：
10.1016/j.envpol.2022.120980
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发表时间：
2023-01-07
影响因子：
8.9
通讯作者：
Sang，Nan
中科院分区：
文献类型：
--
作者：
Yue，Huifeng;Yang，Xiaowen;Sang，Nan
双酚A（BPA）的禁令导致BPA类似物的使用迅速增加，并且它们越来越多地在自然环境和生物有机体中被检测到。研究指出，BPA 类似物可能会导致不良的健康结果。然而，它们对卵巢组织的干扰尚未完全阐明。在这项研究中，7至8周龄的CD-1小鼠通过口服灌胃方式暴露于含有300μg/kg/天双酚B（BPB）或双酚AF（BPAF）的玉米油，并在暴露14天和28天时收集卵巢组织。通过卵巢指数、卵巢面积和卵泡数评估卵巢毒性。 mRNA-seq用于识别差异表达基因（DEG）并推断DEG与卵巢疾病的关联。 BPB 或 BPAF 暴露引起 CD-1 小鼠卵巢组织的形态变化。此外，基因本体（GO）分析揭示了与类固醇生物合成过程（GO：0006694）和细胞钙离子稳态（GO：0006874）相关的生物过程（BP）紊乱。随后，构建了BPA类似物（BPB或BPAF）-DEGs-卵巢疾病的调控网络。重要的是，与卵巢疾病相关的 DEG 和转录因子 (TF) 的表达水平发生了改变。 BPB 或 BPAF 暴露通过多种生物过程的协同作用对卵巢形态造成损害，并且可能与作为卵巢疾病危险因素的 mRNA 表达谱改变有关。
The ban on bisphenol A (BPA) has led to a rapid increase in the use of BPA analogs, and they are increasingly being detected in the natural environment and biological organisms. Studies have pointed out that BPA analogs can lead to adverse health outcomes. However, their interference with ovarian tissue has not been fully elucidated. In this study, seven- to eight-week-old CD-1 mice were exposed to corn oil containing 300 μg/kg/day bisphenol B (BPB) or bisphenol AF (BPAF) through oral gavage, and ovarian tissues were collected at 14 and 28 days of exposure. Ovarian toxicity was evaluated by the ovarian index, ovarian area, and follicle number. mRNA-seq was used to identify differentially expressed genes (DEGs) and infer the association of DEGs with ovarian diseases. BPB or BPAF exposure induced morphological changes in ovarian tissue in CD-1 mice. In addition, Gene Ontology (GO) analysis revealed disturbances in biological processes (BP) associated withsteroid biosynthetic process(GO:0006694) andcellularcalcium ionhomeostasis(GO:0006874). Subsequently, regulatory networks of BPA analogs (BPB or BPAF)-DEGs-ovarian diseases were constructed. Importantly, the expression levels of DEGs and transcription factors (TFs) associated with ovarian disease were altered. BPB or BPAF exposure causes damage to ovarian morphology through the synergistic effects of multiple biological processes and may be associated with altered mRNA expression profiles as a risk factor for ovarian diseases.