Isolation and Characterization of Sweat Gland Myoepithelial Cells from Human Skin
Isolation and Characterization of Sweat Gland Myoepithelial Cells from Human Skin
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DOI：
10.1247/csf.14009
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发表时间：
2014-01-01
影响因子：
1.5
通讯作者：
Sekiguchi, Kiyotoshi
中科院分区：
文献类型：
--
作者：
Kurata, Ryuichiro;Futaki, Sugiko;Sekiguchi, Kiyotoshi
干细胞通常维持主要的表皮成分，即。e.毛囊间表皮、毛囊和汗腺。人体的汗腺遍布全身，是主要通过出汗来调节体温的腺体外分泌器官。新出现的证据表明，干细胞存在于汗腺中，但尚不清楚这种干细胞是否存在于人类汗腺中。在这里，我们试图收集人类汗腺中干细胞的证据，其特征是自我更新能力和多能性。首先，我们探索了人类汗腺细胞干细胞标志物的表达。CD 29和Notch，表皮干细胞标志物，被发现存在于人汗腺中的α-平滑肌肌动蛋白阳性肌上皮细胞中。接着，从人皮肤分离汗腺肌上皮细胞作为CD 29（hi）CD 49 f（hi）亚群。肌上皮细胞富集的CD 29（hi）CD 49 f（hi）亚群具有在球体形成试验中分化为汗腺腔细胞的能力。此外，CD 29（hi）CD 49 f（hi）亚群衍生的球形成细胞在多次传代后表现出长期增殖潜力，表明CD 29（hi）CD 49 f（hi）肌上皮亚群包括具有自我更新能力的干细胞。这些发现提供了证据表明，人类汗腺肌上皮细胞含有干细胞，具有自我更新能力和分化成汗腺的多能性。
Stem cells routinely maintain the main epidermal components, i. e. the interfollicular epidermis, hair follicles, and sweat glands. Human sweat glands present throughout the body are glandular exocrine organs that mainly play a role in thermoregulation by sweating. Emerging evidence points to the presence of stem cells in sweat glands, but it remains unclear whether such stem cells exist in human sweat glands. Here, we attempted to gather evidence for stem cells in human sweat glands, which would be characterized by self-renewal ability and multipotency. First, we explored human sweat gland cells for expression of stem cell markers. CD29 and Notch, epidermal stem cell markers, were found to reside among alpha-smooth muscle actin-positive myoepithelial cells in human sweat glands. Next, sweat gland myoepithelial cells were isolated from human skin as a CD29(hi)CD49f(hi) subpopulation. The myoepithelial cell-enriched CD29(hi)CD49f(hi) subpopulation possessed the ability to differentiate into sweat gland luminal cells in sphere-forming assays. Furthermore, CD29(hi)CD49f(hi) subpopulation-derived sphere-forming cells exhibited long-term proliferative potential upon multiple passaging, indicating that the CD29(hi)CD49f(hi) myoepithelial subpopulation includes stem cells with self-renewal ability. These findings provide evidence that human sweat gland myoepithelial cells contain stem cells that possess both self-renewal ability and multipotency to differentiate into sweat glands.