Polymorphisms of clip domain serine proteinase and serine proteinase homolog in the swimming crab Portunus trituberculatus and their association with Vibrio alginolyticus
Polymorphisms of clip domain serine proteinase and serine proteinase homolog in the swimming crab Portunus trituberculatus and their association with Vibrio alginolyticus
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三疣梭子蟹夹域丝氨酸蛋白酶及丝氨酸蛋白酶同源物多态性及其与溶藻弧菌的关联
DOI：
10.1007/s00343-016-5331-7
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发表时间：
2017-02
期刊：
影响因子：
--
通讯作者：
Cui Zhaoxia
中科院分区：
文献类型：
--
作者：
Liu Yuan;Hui Min;Song chengwen;Cui Zhaoxia
Clip结构域丝氨酸蛋白酶及其同源物在多种生物过程中发挥着重要作用，这些过程是细胞外信号级联反应的重要组成部分，特别是在无脊椎动物的先天免疫反应中。本研究对三瘤梭子蟹PtcSP和PtSPH基因的多态性进行了研究，以探讨它们与溶藻弧菌抗性/敏感性的关系。用Clustal X软件对多态位点进行鉴定，用SPSS16.0软件进行特征分析，用χ-2检验分析抗性群体与感病群体间的基因型和等位基因频率的差异。在PtcSP和PtSPH基因组片段中分别检测到109和77个单核苷酸多态(SNPs)。值得注意的是，近一半的PtSPH多态存在于非编码外显子1。所研究的14个SNP与TOV的易感性/抗药性显著相关。溶藻(P<；0.05)。其中，在内含子中发现了8个SNPs，在编码外显子中发现了1个同义、4个非同义SNPs和1个Indel。此外，在PtcSP基因内含子3还检测到5个简单序列重复序列(SSR)。虽然等位基因频率在统计上没有显著差异，但根据抗性群体和感病群体之间的重复数，SSR表现出不同的多态等位基因。在进一步验证后，本研究的多态性可用于筛选潜在的分子标记OFP。具有抗药性的三瘤菌。溶藻胶菌。
Clip domain serine proteases (cSPs) and their homologs (SPHs) play an important role in various biological processes that are essential components of extracellular signaling cascades, especially in the innate immune responses of invertebrates. Here, polymorphisms of PtcSP and PtSPH from the swimming crabPortunus trituberculatuswere investigated to explore their association with resistance/susceptibility toVibrio alginolyticus. Polymorphic loci were identified using Clustal X, and characterized with SPSS 16.0 software, and then the significance of genotype and allele frequencies between resistant and susceptible stocks was determined by aχ2test. A total of 109 and 77 single nucleotide polymorphisms (SNPs) were identified in the genomic fragments of PtcSP and PtSPH, respectively. Notably, nearly half of PtSPH polymorphisms were found in the non-coding exon 1. Fourteen SNPs investigated were significantly associated with susceptibility/resistance toV. alginolyticus(P<0.05). Among them, eight SNPs were observed in introns, and one synonymous, four non-synonymous SNPs and one ins-del were found in coding exons. In addition, five simple sequence repeats (SSRs) were detected in intron 3 of PtcSP. Although there was no statistically significant difference of allele frequencies, the SSRs showed different polymorphic alleles on the basis of the repeat number between resistant and susceptible stocks. After further validation, polymorphisms investigated here might be applied to select potential molecular markers ofP. trituberculatuswith resistance toV. alginolyticus.
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影响因子：
2
作者：
Chengwen Song;Danli Luo;Z. Cui;Yuan Liu;Xihong Li;Guohui Shi;Chunlin Wang
通讯作者：
Chengwen Song;Danli Luo;Z. Cui;Yuan Liu;Xihong Li;Guohui Shi;Chunlin Wang
使用非同义/同义率比率分析正选位点的变化：使用流感病毒的示例。
影响因子：
3.7
作者：
Chen J;Sun Y
通讯作者：
Sun Y
影响因子：
3.6
作者：
Lan-Ju Wang;Jianguo Su;Chunrong Yang;Q. Wan;Limin Peng
通讯作者：
Lan-Ju Wang;Jianguo Su;Chunrong Yang;Q. Wan;Limin Peng
影响因子：
4.7
作者：
Chengwen Song;Z. Cui;Yuan Liu;Qianqian Li;Xihong Li;Guohui Shi;Chunlin Wang
通讯作者：
Chengwen Song;Z. Cui;Yuan Liu;Qianqian Li;Xihong Li;Guohui Shi;Chunlin Wang
影响因子：
3.9
作者：
John M. Hancock
通讯作者：
John M. Hancock