Use of synovial fluid markers of cartilage synthesis and turnover to study effects of repeated intra-articular administration of methylprednisolone acetate on articular cartilage in vivo
Use of synovial fluid markers of cartilage synthesis and turnover to study effects of repeated intra-articular administration of methylprednisolone acetate on articular cartilage in vivo
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DOI：
10.1016/s0736-0266(00)90008-1
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发表时间：
2001-03-01
影响因子：
2.8
通讯作者：
Laverty, S
中科院分区：
文献类型：
--
作者：
Robion, FC;Doizé, B;Laverty, S
在体内，关节内（IA）皮质类固醇对关节软骨的影响仍然存在争议。本研究的目的是检查这个问题，使用滑液（SF）标记的软骨代谢配对桡腕关节。没有关节疾病的临床或影像学体征，在10匹成年马中进行了研究。每周进行一次无菌关节穿刺术，持续13周。在第3、5和7周，将醋酸甲泼尼龙（MPA）IA注射到治疗关节中，并将溶剂IA注射到对照关节中。我们对SF样品使用放射免疫测定，其测量硫酸角质素表位（KS）和软骨聚集蛋白聚糖（PG）上的846表位以及软骨II型前胶原的C-前肽（CPII），所述C-前肽在该分子合成后释放。对选定的SF样品进行凝胶色谱以评价SF PG分子的大小。总关节KS和846个表位都存在于主要分子的异质性群体上，从色谱分析，似乎主要是关节软骨聚集蛋白聚糖的片段。在治疗期间，与对照组相比，MPA关节中它们显著升高，而CPII mas显著降低。这些结果表明，反复使用IA MPA导致对II型前胶原合成的潜在有害抑制和PG聚集蛋白聚糖降解产物从关节软骨的释放增加。(C)2001骨科研究学会。由Elsevier Sciene Ltd.出版。保留所有权利。
In vivo the effects of intra-articular (IA) corticosteroids on articular cartilage remain controversial. This study was designed to examine this issue using synovial fluid (SF) markers of cartilage metabolism Paired radiocarpal joints. without clinical or radiographic signs of joint disease, were studied in 10 adult horses. Aseptic arthrocentesis was performed weekly for 13 weeks. IA injections of methylprednisolone acetate (MPA) into the treatment joint and the vehicle into the control joint were performed at weeks 3, 5 and 7. We used radioimmunoassays on SF samples which measure a keratan sulfate epitope (KS) and the 846 epitope on cartilage aggrecan (PG) and the C-propeptile (CPII) of cartilage type II procollagen which is released following synthesis of this molecule. Gel chromatography was performed on selected SF samples to evaluate the sizes of SF PG molecules. The total joint KS and the 846 epitopes were both present on a heterogeneous population of mainly molecules which, from chromotographic analysis, appeared to be mainly fragments of the articular cartilage aggrecan. They were significantly elevated in MPA joints whereas CPII mas significantly reduced compared to the control during the treatment period. These results indicate that the repeated use of IA MPA leads to a potentially harmful inhibition of procollagen II synthesis and an increased release of degradation products of the PG aggrecan from articular cartilage. (C) 2001 Orthopaedic Research Society. Published by Elsevier Sciene Ltd. All rights reserved.